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@ARTICLE{Tiso:892391,
      author       = {Tiso, Till and Narancic, Tanja and Wei, Ren and Pollet,
                      Eric and Beagan, Niall and Schröder, Katja and Honak,
                      Annett and Jiang, Mengying and Kenny, Shane T. and Wierckx,
                      Nick and Perrin, Rémi and Avérous, Luc and Zimmermann,
                      Wolfgang and O'Connor, Kevin and Blank, Lars M.},
      title        = {{T}owards bio-upcycling of polyethylene terephthalate},
      journal      = {Metabolic engineering},
      volume       = {66},
      issn         = {1096-7176},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2021-02052},
      pages        = {167 - 178},
      year         = {2021},
      note         = {Biotechnologie 1},
      abstract     = {Over 359 million tons of plastics were produced worldwide
                      in 2018, with significant growth expected in the near
                      future, resulting in the global challenge of end-of-life
                      management. The recent identification of enzymes that
                      degrade plastics previously considered non-biodegradable
                      opens up opportunities to steer the plastic recycling
                      industry into the realm of biotechnology.Here, the
                      sequential conversion of post-consumer polyethylene
                      terephthalate (PET) into two types of bioplastics is
                      presented: a medium chain-length polyhydroxyalkanoate (PHA)
                      and a novel bio-based poly(amide urethane) (bio-PU). PET
                      films are hydrolyzed by a thermostable polyester hydrolase
                      yielding highly pure terephthalate and ethylene glycol. The
                      obtained hydrolysate is used directly as a feedstock for a
                      terephthalate-degrading Pseudomonas umsongensis GO16, also
                      evolved to efficiently metabolize ethylene glycol, to
                      produce PHA. The strain is further modified to secrete
                      hydroxyalkanoyloxy-alkanoates (HAAs), which are used as
                      monomers for the chemo-catalytic synthesis of bio-PU. In
                      short, a novel value-chain for PET upcycling is shown that
                      circumvents the costly purification of PET monomers, adding
                      technological flexibility to the global challenge of
                      end-of-life management of plastics.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33865980},
      UT           = {WOS:000658808700005},
      doi          = {10.1016/j.ymben.2021.03.011},
      url          = {https://juser.fz-juelich.de/record/892391},
}