% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Ackermann:892434,
      author       = {Ackermann, Yannic S. and Li, Wing-Jin and Op de Hipt,
                      Leonie and Niehoff, Paul-Joachim and Casey, William and
                      Polen, Tino and Köbbing, Sebastian and Ballerstedt, Hendrik
                      and Wynands, Benedikt and O'Connor, Kevin and Blank, Lars M.
                      and Wierckx, Nick},
      title        = {{E}ngineering adipic acid metabolism in {P}seudomonas
                      putida},
      journal      = {Metabolic engineering},
      volume       = {67},
      issn         = {1096-7176},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2021-02080},
      pages        = {29-40},
      year         = {2021},
      note         = {Biotechnologie 1},
      abstract     = {Bio-upcycling of plastics is an upcoming alternative
                      approach for the valorization of diverse polymer waste
                      streams that are too contaminated for traditional recycling
                      technologies. Adipic acid and other medium-chain-length
                      dicarboxylates are key components of many plastics including
                      polyamides, polyesters, and polyurethanes. This study endows
                      Pseudomonas putida KT2440 with efficient metabolism of these
                      dicarboxylates. The dcaAKIJP genes from Acinetobacter
                      baylyi, encoding initial uptake and activation steps for
                      dicarboxylates, were heterologously expressed. Genomic
                      integration of these dca genes proved to be a key factor in
                      efficient and reliable expression. In spite of this,
                      adaptive laboratory evolution was needed to connect these
                      initial steps to the native metabolism of P. putida, thereby
                      enabling growth on adipate as sole carbon source. Genome
                      sequencing of evolved strains revealed a central role of a
                      paa gene cluster, which encodes parts of the phenylacetate
                      metabolic degradation pathway with parallels to adipate
                      metabolism. Fast growth required the additional disruption
                      of the regulator-encoding psrA, which upregulates redundant
                      β-oxidation genes. This knowledge enabled the rational
                      reverse engineering of a strain that can not only use
                      adipate, but also other medium-chain-length dicarboxylates
                      like suberate and sebacate. The reverse engineered strain
                      grows on adipate with a rate of 0.35 ± 0.01 h−1,
                      reaching a final biomass yield of 0.27 ± 0.00 gCDW
                      gadipate−1. In a nitrogen-limited medium this strain
                      produced polyhydroxyalkanoates from adipate up to $25\%$ of
                      its CDW. This proves its applicability for the upcycling of
                      mixtures of polymers made from fossile resources into
                      biodegradable counterparts.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33965615},
      UT           = {WOS:000694909400004},
      doi          = {10.1016/j.ymben.2021.05.001},
      url          = {https://juser.fz-juelich.de/record/892434},
}