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000892863 020__ $$a978-3-95806-597-0
000892863 037__ $$aFZJ-2021-02402
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000892863 1001_ $$0P:(DE-Juel1)162348$$aTihaa, Irina$$b0$$eCorresponding author$$gfemale$$ufzj
000892863 245__ $$aEngineering neuronal networks in vitro: From single cells to population connectivity$$f2014-03-31 - 2018-09-30
000892863 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2021
000892863 300__ $$aviii, 242 S.
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000892863 4900_ $$aSchriften des Forschungszentrums Jülich. Reihe Information / Information$$v78
000892863 502__ $$aRWTH Aachen, Diss., 2021$$bDissertation$$cRWTH Aachen$$d2021$$o2021-06-21
000892863 520__ $$aThe mammalian brain shows multiple levels of organisation ranging from single cell to regional organisation level. Structure and function are dependent on each other. The aim of this thesis is to incorporate the essential feature of organisation into cultured neuronal networks via microcontact printing (µCP). We do so to better understand the impact of pattern geometry onto network architecture, extend the $\textit{in vitro}$ models and provide more relevant systems. The first part of this thesis considers single cell connectivity. Grid and star pattern designs featuring nodes and lines are utilized to control soma position and neurite elongation. Morphological analysis via immunouorescence and live cell imaging revealed a high dependence of soma position and axon guiding efficiency on the patterns. For soma position itself the shape, dimensions and proportions of the pattern features are of great importance. Calcium imaging and electrical recordings with multi-electrode arrays (MEAs) proved functional connectivity of the created single cell networks.In the second part, triangular shaped structures were used to create modular neuronal networks with differently sized populations. Immunofluorescence analysis revealed a highly directional structural connectivity between the populations. Calcium imaging analyses demonstrated a high intra-population and a weaker inter-population interconnectedness, a typical feature of modularly organised networks. Moreover, synchrony was observed to decrease with increasing population size indicating a rise in architectural complexity. Overall, this thesis illustrates the potential of µCP for designing $\textit{in vitro}$ neuronal networks with micro- to mesoscale level of organisation. Here, the pattern design is crucial for the network architecture. A deeper understanding of the impact of pattern geometry onto network formation might contribute to a greater use of defined networks for neurobiological experiments by enhancing efficiency and predictability of patterned cultures. [...]
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000892863 9141_ $$y2021
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000892863 9130_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
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