TY  - JOUR
AU  - Apetz, Nadine
AU  - Paralikar, Kunal
AU  - Neumaier, Bernd
AU  - Drzezga, Alexander
AU  - Wiedermann, Dirk
AU  - Iyer, Rajesh
AU  - Munns, Gordon
AU  - Scott, Erik
AU  - Timmermann, Lars
AU  - Endepols, Heike
TI  - Towards chronic deep brain stimulation in freely moving hemiparkinsonian rats: applicability and functionality of a fully implantable stimulation system
JO  - Journal of neural engineering
VL  - 18
IS  - 3
SN  - 1741-2552
CY  - Bristol
PB  - Institute of Physics Publishing
M1  - FZJ-2021-02548
SP  - 036018
PY  - 2021
AB  - This study aimed at investigating a novel fully implantable deep brain stimulation(DBS) system and its ability to modulate brain metabolism and behavior through subthalamicnucleus (STN) stimulation in a hemiparkinsonian rat model.Approach.Twelve male rats wereunilaterally lesioned with 6-hydroxydopamine in the medial forebrain bundle and received a fullyimplantable DBS system aiming at the ipsilesional STN. Each rat underwent three cylinder tests toanalyze front paw use: a PRE test before any surgical intervention, an OFF test after surgery butbefore stimulation onset and an ON test under DBS. To visualize brain glucose metabolism in theawake animal, two [18F]FDG scans were conducted in the OFF and ON condition. At least 4 weeksafter surgery, an [18F]FDOPA scan was used to check for dopaminergic integrity.Main results.Ingeneral, STN DBS increased [18F]FDG uptake ipsilesionally and decreased it contralesionally. Morespecifically, bilateral orbitofrontal cortex, ipsilateral caudate putamen, sensorimotor cortex andnucleus accumbens showed significantly higher tracer uptake in ON compared to OFF condition.Contralateral cingulate and secondary motor cortex, caudate putamen, amygdala, hippocampus,retrosplenial granular cortex, superior colliculus, and parts of the cerebellum exhibitedsignificantly higher [18F]FDG uptake in the OFF condition. On the behavioral level, stimulationwas able improve use of the contralesional affected front paw suggesting an effective stimulationproduced by the implanted system.Significance.The fully implantable stimulation systemdeveloped by us and presented here offers the output of arbitrary user-defined waveforms, patternsand stimulation settings and allows tracer accumulation in freely moving animals. It is therefore asuitable device for implementing behavioral PET studies. It contributes immensely to thepossibilities to characterize and unveil the effects and mechanisms of DBS offering valuable cluesfor future improvements of this therapy
LB  - PUB:(DE-HGF)16
C6  - 33607640
UR  - <Go to ISI:>//WOS:000629524500001
DO  - DOI:10.1088/1741-2552/abe806
UR  - https://juser.fz-juelich.de/record/893080
ER  -