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@ARTICLE{Apetz:893080,
author = {Apetz, Nadine and Paralikar, Kunal and Neumaier, Bernd and
Drzezga, Alexander and Wiedermann, Dirk and Iyer, Rajesh and
Munns, Gordon and Scott, Erik and Timmermann, Lars and
Endepols, Heike},
title = {{T}owards chronic deep brain stimulation in freely moving
hemiparkinsonian rats: applicability and functionality of a
fully implantable stimulation system},
journal = {Journal of neural engineering},
volume = {18},
number = {3},
issn = {1741-2552},
address = {Bristol},
publisher = {Institute of Physics Publishing},
reportid = {FZJ-2021-02548},
pages = {036018},
year = {2021},
abstract = {This study aimed at investigating a novel fully implantable
deep brain stimulation(DBS) system and its ability to
modulate brain metabolism and behavior through
subthalamicnucleus (STN) stimulation in a hemiparkinsonian
rat model.Approach.Twelve male rats wereunilaterally
lesioned with 6-hydroxydopamine in the medial forebrain
bundle and received a fullyimplantable DBS system aiming at
the ipsilesional STN. Each rat underwent three cylinder
tests toanalyze front paw use: a PRE test before any
surgical intervention, an OFF test after surgery butbefore
stimulation onset and an ON test under DBS. To visualize
brain glucose metabolism in theawake animal, two [18F]FDG
scans were conducted in the OFF and ON condition. At least 4
weeksafter surgery, an [18F]FDOPA scan was used to check for
dopaminergic integrity.Main results.Ingeneral, STN DBS
increased [18F]FDG uptake ipsilesionally and decreased it
contralesionally. Morespecifically, bilateral orbitofrontal
cortex, ipsilateral caudate putamen, sensorimotor cortex
andnucleus accumbens showed significantly higher tracer
uptake in ON compared to OFF condition.Contralateral
cingulate and secondary motor cortex, caudate putamen,
amygdala, hippocampus,retrosplenial granular cortex,
superior colliculus, and parts of the cerebellum
exhibitedsignificantly higher [18F]FDG uptake in the OFF
condition. On the behavioral level, stimulationwas able
improve use of the contralesional affected front paw
suggesting an effective stimulationproduced by the implanted
system.Significance.The fully implantable stimulation
systemdeveloped by us and presented here offers the output
of arbitrary user-defined waveforms, patternsand stimulation
settings and allows tracer accumulation in freely moving
animals. It is therefore asuitable device for implementing
behavioral PET studies. It contributes immensely to
thepossibilities to characterize and unveil the effects and
mechanisms of DBS offering valuable cluesfor future
improvements of this therapy},
cin = {INM-5 / INM-2},
ddc = {610},
cid = {I:(DE-Juel1)INM-5-20090406 / I:(DE-Juel1)INM-2-20090406},
pnm = {525 - Decoding Brain Organization and Dysfunction
(POF4-525)},
pid = {G:(DE-HGF)POF4-525},
typ = {PUB:(DE-HGF)16},
pubmed = {33607640},
UT = {WOS:000629524500001},
doi = {10.1088/1741-2552/abe806},
url = {https://juser.fz-juelich.de/record/893080},
}