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@ARTICLE{Prescher:893209,
author = {Prescher, Martin and Bonus, Michele and Stindt, Jan and
Keitel-Anselmino, Verena and Smits, Sander H. J. and Gohlke,
Holger and Schmitt, Lutz},
title = {{E}vidence for a credit-card-swipe mechanism in the human
{PC} floppase {ABCB}4},
journal = {Structure},
volume = {29},
number = {10},
issn = {0969-2126},
address = {Cambridge, Mass.},
publisher = {Cell Press},
reportid = {FZJ-2021-02626},
pages = {1144-1155.e5},
year = {2021},
abstract = {ABCB4 is described as an ATP-binding cassette (ABC)
transporter that primarily transports lipids of the
phosphatidylcholine (PC) family but is also capable of
translocating a subset of typical
multidrug-resistance-associated drugs. The high degree of
amino acid identity of $76\%$ for ABCB4 and ABCB1, which is
a prototype multidrug-resistance-mediating protein, results
in ABCB4's second subset of substrates, which overlap with
ABCB1's substrates. This often leads to incomplete
annotations of ABCB4, in which it was described as
exclusively PC-lipid specific. When the hydrophilic amino
acids from ABCB4 are changed to the analogous but
hydrophobic ones from ABCB1, the stimulation of ATPase
activity by 1,2-dioleoyl-sn-glycero-3-phosphocholine, as a
prime example of PC lipids, is strongly diminished, whereas
the modulation capability of ABCB1 substrates remains
unchanged. This indicates two distinct and autonomous
substrate binding sites in ABCB4.},
cin = {IBG-4 / NIC / JSC / IBI-7},
ddc = {540},
cid = {I:(DE-Juel1)IBG-4-20200403 / I:(DE-Juel1)NIC-20090406 /
I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)IBI-7-20200312},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511) / 2171 - Biological and environmental resources
for sustainable use (POF4-217) / 2172 - Utilization of
renewable carbon and energy sources and engineering of
ecosystem functions (POF4-217) / 5111 - Domain-Specific
Simulation $\&$ Data Life Cycle Labs (SDLs) and Research
Groups (POF4-511) / Forschergruppe Gohlke $(hkf7_20200501)$},
pid = {G:(DE-HGF)POF3-511 / G:(DE-HGF)POF4-2171 /
G:(DE-HGF)POF4-2172 / G:(DE-HGF)POF4-5111 /
$G:(DE-Juel1)hkf7_20200501$},
typ = {PUB:(DE-HGF)16},
pubmed = {34107287},
UT = {WOS:000743716700006},
doi = {10.1016/j.str.2021.05.013},
url = {https://juser.fz-juelich.de/record/893209},
}