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@ARTICLE{Deutsch:893729,
      author       = {Deutsch, Matthias and Stegmayr, Carina and Balfanz, Sabine
                      and Baumann, Arnd},
      title        = {{L}oss of {HCN}2 in {D}orsal {H}ippocampus of {Y}oung
                      {A}dult {M}ice {I}nduces {S}pecific {A}poptosis of the {CA}1
                      {P}yramidal {N}euron {L}ayer},
      journal      = {International journal of molecular sciences},
      volume       = {22},
      number       = {13},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2021-02784},
      pages        = {6699 -},
      year         = {2021},
      abstract     = {Neurons inevitably rely on a proper repertoire and
                      distribution of membrane-bound ion-conducting channels.
                      Among these proteins, the family of
                      hyperpolarization-activated and cyclic nucleotide-gated
                      (HCN) channels possesses unique properties giving rise to
                      the corresponding Ih-current that contributes to various
                      aspects of neural signaling. In mammals, four genes (hcn1-4)
                      encode subunits of HCN channels. These subunits can assemble
                      as hetero- or homotetrameric ion-conducting channels. In
                      order to elaborate on the specific role of the HCN2 subunit
                      in shaping electrical properties of neurons, we applied an
                      Adeno-associated virus (AAV)-mediated, RNAi-based knock-down
                      strategy of hcn2 gene expression both in vitro and in vivo.
                      Electrophysiological measurements showed that HCN2 subunit
                      knock-down resulted in specific yet anticipated changes in
                      Ih-current properties in primary hippocampal neurons and, in
                      addition, corroborated that the HCN2 subunit participates in
                      postsynaptic signal integration. To further address the role
                      of the HCN2 subunit in vivo, we injected recombinant (r)AAVs
                      into the dorsal hippocampus of young adult male mice.
                      Behavioral and biochemical analyses were conducted to assess
                      the contribution of HCN2-containing channels in shaping
                      hippocampal network properties. Surprisingly, knock-down of
                      hcn2 expression resulted in a severe degeneration of the CA1
                      pyramidal cell layer, which did not occur in mice injected
                      with control rAAV constructs. This finding might pinpoint to
                      a vital and yet unknown contribution of HCN2 channels in
                      establishing or maintaining the proper function of CA1
                      pyramidal neurons of the dorsal hippocampus.},
      cin          = {IBI-1 / INM-4},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-1-20200312 / I:(DE-Juel1)INM-4-20090406},
      pnm          = {5243 - Information Processing in Distributed Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5243},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34206649},
      UT           = {WOS:000670928800001},
      doi          = {10.3390/ijms22136699},
      url          = {https://juser.fz-juelich.de/record/893729},
}