%0 Journal Article
%A Couto, Joao
%A Musall, Simon
%A Sun, Xiaonan R.
%A Khanal, Anup
%A Gluf, Steven
%A Saxena, Shreya
%A Kinsella, Ian
%A Abe, Taiga
%A Cunningham, John P.
%A Paninski, Liam
%A Churchland, 9and Anne K.
%T Chronic, cortex-wide imaging of specific cell populations during behavior
%J Nature protocols
%V 16
%@ 1750-2799
%C Basingstoke
%I Nature Publishing Group
%M FZJ-2021-02792
%P 3241–3263
%D 2021
%X Measurements of neuronal activity across brain areas are important for understanding the neural correlates of cognitive and motor processes such as attention, decision-making and action selection. However, techniques that allow cellular resolution measurements are expensive and require a high degree of technical expertise, which limits their broad use. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective and flexible approach to measure activity of specific cell populations with high temporal resolution and a cortex-wide field of view. Here we outline our protocol for assembling a wide-field macroscope setup, performing surgery to prepare the intact skull and imaging neural activity chronically in behaving, transgenic mice. Further, we highlight a processing pipeline that leverages novel, cloud-based methods to analyze large-scale imaging datasets. The protocol targets laboratories that are seeking to build macroscopes, optimize surgical procedures for long-term chronic imaging and/or analyze cortex-wide neuronal recordings. The entire protocol, including steps for assembly and calibration of the macroscope, surgical preparation, imaging and data analysis, requires a total of 8 h. It is designed to be accessible to laboratories with limited expertise in imaging methods or interest in high-throughput imaging during behavior.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 34075229
%U <Go to ISI:>//WOS:000656804600002
%R 10.1038/s41596-021-00527-z
%U https://juser.fz-juelich.de/record/893737