TY  - JOUR
AU  - Couto, Joao
AU  - Musall, Simon
AU  - Sun, Xiaonan R.
AU  - Khanal, Anup
AU  - Gluf, Steven
AU  - Saxena, Shreya
AU  - Kinsella, Ian
AU  - Abe, Taiga
AU  - Cunningham, John P.
AU  - Paninski, Liam
AU  - Churchland, 9and Anne K.
TI  - Chronic, cortex-wide imaging of specific cell populations during behavior
JO  - Nature protocols
VL  - 16
SN  - 1750-2799
CY  - Basingstoke
PB  - Nature Publishing Group
M1  - FZJ-2021-02792
SP  - 3241–3263
PY  - 2021
AB  - Measurements of neuronal activity across brain areas are important for understanding the neural correlates of cognitive and motor processes such as attention, decision-making and action selection. However, techniques that allow cellular resolution measurements are expensive and require a high degree of technical expertise, which limits their broad use. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective and flexible approach to measure activity of specific cell populations with high temporal resolution and a cortex-wide field of view. Here we outline our protocol for assembling a wide-field macroscope setup, performing surgery to prepare the intact skull and imaging neural activity chronically in behaving, transgenic mice. Further, we highlight a processing pipeline that leverages novel, cloud-based methods to analyze large-scale imaging datasets. The protocol targets laboratories that are seeking to build macroscopes, optimize surgical procedures for long-term chronic imaging and/or analyze cortex-wide neuronal recordings. The entire protocol, including steps for assembly and calibration of the macroscope, surgical preparation, imaging and data analysis, requires a total of 8 h. It is designed to be accessible to laboratories with limited expertise in imaging methods or interest in high-throughput imaging during behavior.
LB  - PUB:(DE-HGF)16
C6  - 34075229
UR  - <Go to ISI:>//WOS:000656804600002
DO  - DOI:10.1038/s41596-021-00527-z
UR  - https://juser.fz-juelich.de/record/893737
ER  -