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@ARTICLE{Couto:893737,
      author       = {Couto, Joao and Musall, Simon and Sun, Xiaonan R. and
                      Khanal, Anup and Gluf, Steven and Saxena, Shreya and
                      Kinsella, Ian and Abe, Taiga and Cunningham, John P. and
                      Paninski, Liam and Churchland, 9and Anne K.},
      title        = {{C}hronic, cortex-wide imaging of specific cell populations
                      during behavior},
      journal      = {Nature protocols},
      volume       = {16},
      issn         = {1750-2799},
      address      = {Basingstoke},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2021-02792},
      pages        = {3241–3263},
      year         = {2021},
      abstract     = {Measurements of neuronal activity across brain areas are
                      important for understanding the neural correlates of
                      cognitive and motor processes such as attention,
                      decision-making and action selection. However, techniques
                      that allow cellular resolution measurements are expensive
                      and require a high degree of technical expertise, which
                      limits their broad use. Wide-field imaging of genetically
                      encoded indicators is a high-throughput, cost-effective and
                      flexible approach to measure activity of specific cell
                      populations with high temporal resolution and a cortex-wide
                      field of view. Here we outline our protocol for assembling a
                      wide-field macroscope setup, performing surgery to prepare
                      the intact skull and imaging neural activity chronically in
                      behaving, transgenic mice. Further, we highlight a
                      processing pipeline that leverages novel, cloud-based
                      methods to analyze large-scale imaging datasets. The
                      protocol targets laboratories that are seeking to build
                      macroscopes, optimize surgical procedures for long-term
                      chronic imaging and/or analyze cortex-wide neuronal
                      recordings. The entire protocol, including steps for
                      assembly and calibration of the macroscope, surgical
                      preparation, imaging and data analysis, requires a total of
                      8 h. It is designed to be accessible to laboratories with
                      limited expertise in imaging methods or interest in
                      high-throughput imaging during behavior.},
      cin          = {IBI-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5231 - Neuroscientific Foundations (POF4-523)},
      pid          = {G:(DE-HGF)POF4-5231},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34075229},
      UT           = {WOS:000656804600002},
      doi          = {10.1038/s41596-021-00527-z},
      url          = {https://juser.fz-juelich.de/record/893737},
}