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| 001 | 893737 | ||
| 005 | 20210810182032.0 | ||
| 024 | 7 | _ | |a 10.1038/s41596-021-00527-z |2 doi |
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| 100 | 1 | _ | |a Couto, Joao |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Chronic, cortex-wide imaging of specific cell populations during behavior |
| 260 | _ | _ | |a Basingstoke |c 2021 |b Nature Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Measurements of neuronal activity across brain areas are important for understanding the neural correlates of cognitive and motor processes such as attention, decision-making and action selection. However, techniques that allow cellular resolution measurements are expensive and require a high degree of technical expertise, which limits their broad use. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective and flexible approach to measure activity of specific cell populations with high temporal resolution and a cortex-wide field of view. Here we outline our protocol for assembling a wide-field macroscope setup, performing surgery to prepare the intact skull and imaging neural activity chronically in behaving, transgenic mice. Further, we highlight a processing pipeline that leverages novel, cloud-based methods to analyze large-scale imaging datasets. The protocol targets laboratories that are seeking to build macroscopes, optimize surgical procedures for long-term chronic imaging and/or analyze cortex-wide neuronal recordings. The entire protocol, including steps for assembly and calibration of the macroscope, surgical preparation, imaging and data analysis, requires a total of 8 h. It is designed to be accessible to laboratories with limited expertise in imaging methods or interest in high-throughput imaging during behavior. |
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| 700 | 1 | _ | |a Musall, Simon |0 P:(DE-Juel1)175146 |b 1 |u fzj |
| 700 | 1 | _ | |a Sun, Xiaonan R. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Khanal, Anup |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Gluf, Steven |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Saxena, Shreya |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Kinsella, Ian |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Abe, Taiga |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Cunningham, John P. |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Paninski, Liam |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Churchland, 9and Anne K. |0 P:(DE-HGF)0 |b 10 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/s41596-021-00527-z |0 PERI:(DE-600)2244966-8 |p 3241–3263 |t Nature protocols |v 16 |y 2021 |x 1750-2799 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/893737/files/s41596-021-00527-z.pdf |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/893737/files/2010.15191.pdf |
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