TY  - JOUR
AU  - Zabelskii, Dmitrii
AU  - Dmitrieva, Natalia
AU  - Volkov, Oleksandr
AU  - Shevchenko, Vitaly
AU  - Kovalev, Kirill
AU  - Balandin, Taras
AU  - Soloviov, Dmytro
AU  - Astashkin, Roman
AU  - Zinovev, Egor
AU  - Alekseev, Alexey
AU  - Round, Ekaterina
AU  - Polovinkin, Vitaly
AU  - Chizhov, Igor
AU  - Rogachev, Andrey
AU  - Okhrimenko, Ivan
AU  - Borshchevskiy, Valentin
AU  - Chupin, Vladimir
AU  - Büldt, Georg
AU  - Yutin, Natalia
AU  - Bamberg, Ernst
AU  - Koonin, Eugene
AU  - Gordeliy, Valentin
TI  - Structure-based insights into evolution of rhodopsins
JO  - Communications biology
VL  - 4
IS  - 1
SN  - 2399-3642
CY  - London
PB  - Springer Nature
M1  - FZJ-2021-02817
SP  - 821
PY  - 2021
AB  - Rhodopsins, most of which are proton pumps generating transmembrane electrochemical proton gradients, span all three domains of life, are abundant in the biosphere, and could play a crucial role in the early evolution of life on earth. Whereas archaeal and bacterial proton pumps are among the best structurally characterized proteins, rhodopsins from unicellular eukaryotes have not been well characterized. To fill this gap in the current understanding of the proton pumps and to gain insight into the evolution of rhodopsins using a structure-based approach, we performed a structural and functional analysis of the light-driven proton pump LR (Mac) from the pathogenic fungus Leptosphaeria maculans. The first high-resolution structure of fungi rhodopsin and its functional properties reveal the striking similarity of its membrane part to archaeal but not to bacterial rhodopsins. We show that an unusually long N-terminal region stabilizes the protein through direct interaction with its extracellular loop (ECL2). We compare to our knowledge all available structures and sequences of outward light-driven proton pumps and show that eukaryotic and archaeal proton pumps, most likely, share a common ancestor.
LB  - PUB:(DE-HGF)16
C6  - 34193947
UR  - <Go to ISI:>//WOS:000671710700003
DO  - DOI:10.1038/s42003-021-02326-4
UR  - https://juser.fz-juelich.de/record/893762
ER  -