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@ARTICLE{Bogorodskiy:893931,
      author       = {Bogorodskiy, Andrey and Okhrimenko, Ivan and Maslov, Ivan
                      and Maliar, Nina and Burkatovskii, Dmitrii and von Ameln,
                      Florian and Schulga, Alexey and Jakobs, Philipp and
                      Altschmied, Joachim and Haendeler, Judith and Katranidis,
                      Alexandros and Sorokin, Ivan and Mishin, Alexey and
                      Gordeliy, Valentin and Büldt, Georg and Voos, Wolfgang and
                      Gensch, Thomas and Borshchevskiy, Valentin},
      title        = {{A}ccessing {M}itochondrial {P}rotein {I}mport in {L}iving
                      {C}ells by {P}rotein {M}icroinjection},
      journal      = {Frontiers in cell and developmental biology},
      volume       = {9},
      issn         = {2296-634X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2021-02941},
      pages        = {698658},
      year         = {2021},
      abstract     = {Mitochondrial protein biogenesis relies almost exclusively
                      on the expression of nuclear-encoded polypeptides. The
                      current model postulates that most of these proteins have to
                      be delivered to their final mitochondrial destination after
                      their synthesis in the cytoplasm. However, the knowledge of
                      this process remains limited due to the absence of proper
                      experimental real-time approaches to study mitochondria in
                      their native cellular environment. We developed a gentle
                      microinjection procedure for fluorescent reporter proteins
                      allowing a direct non-invasive study of protein transport in
                      living cells. As a proof of principle, we visualized
                      potential-dependent protein import into mitochondria inside
                      intact cells in real-time. We validated that our approach
                      does not distort mitochondrial morphology and preserves the
                      endogenous expression system as well as mitochondrial
                      protein translocation machinery. We observed that a release
                      of nascent polypeptides chains from actively translating
                      cellular ribosomes by puromycin strongly increased the
                      import rate of the microinjected pre-protein. This suggests
                      that a substantial amount of mitochondrial translocase
                      complexes was involved in co-translational protein import of
                      endogenously expressed pre-proteins. Our protein
                      microinjection method opens new possibilities to study the
                      role of mitochondrial protein import in cell models of
                      various pathological conditions as well as aging processes.},
      cin          = {IBI-1 / IBI-6 / IBI-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-1-20200312 / I:(DE-Juel1)IBI-6-20200312 /
                      I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5243 - Information Processing in Distributed Systems
                      (POF4-524) / 5352 - Understanding the Functionality of Soft
                      Matter and Biomolecular Systems (POF4-535) / 5241 -
                      Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5243 / G:(DE-HGF)POF4-5352 /
                      G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34307376},
      UT           = {WOS:000674898900001},
      doi          = {10.3389/fcell.2021.698658},
      url          = {https://juser.fz-juelich.de/record/893931},
}