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@ARTICLE{Pllen:894173,
author = {Püllen, Robin and Konrad, Jens and Merkel, Rudolf and
Hoffmann, Bernd},
title = {{S}kin under {S}train: {F}rom {E}pithelial {M}odel
{T}issues to {A}dult {E}pithelia},
journal = {Cells},
volume = {10},
number = {7},
issn = {2073-4409},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2021-03075},
pages = {1834 -},
year = {2021},
abstract = {Formation of a barrier capable of protecting tissue from
external damage, chemical factors, and pathogens is one of
the main functions of the epidermis. Furthermore, upon
development and during aging, mechanoprotective epidermal
functions change dramatically. However, comparative studies
between embryonic and adult skin in comparison to skin
equivalents are still scarce which is especially due to the
lack of appropriate measurement systems with sufficient
accuracy and long-term tissue compatibility. Our studies
fill this gap by developing a combined bioreactor and
tensile testing machine for biomechanical analysis of living
epithelia. Based on this tissue stretcher, our data clearly
show that viscoelastic and plastic deformation behavior of
embryonic and adult skin differ significantly. Tissue
responses to static strain compared to cyclic strain also
show a clear dependence on differentiation stage.
Multilayered unkeratinized epidermis equivalents, on the
other hand, respond very similar to mechanical stretch as
adult tissue. This mechanical similarity is even more
evident after a single cycle of mechanical preconditioning.
Our studies therefore suggest that skin equivalents are well
suited model systems to analyze cellular interactions of
epidermal cells in natural tissues},
cin = {IBI-2},
ddc = {570},
cid = {I:(DE-Juel1)IBI-2-20200312},
pnm = {5243 - Information Processing in Distributed Systems
(POF4-524) / DFG project 273723265 - Mechanosensation und
Mechanoreaktion in epidermalen Systemen},
pid = {G:(DE-HGF)POF4-5243 / G:(GEPRIS)273723265},
typ = {PUB:(DE-HGF)16},
pubmed = {34360001},
UT = {WOS:000676829000001},
doi = {10.3390/cells10071834},
url = {https://juser.fz-juelich.de/record/894173},
}