TY  - JOUR
AU  - Wang, Liming
AU  - Amelung, Wulf
AU  - Willbold, Sabine
TI  - 18 O Isotope Labeling Combined with 31 P Nuclear Magnetic Resonance Spectroscopy for Accurate Quantification of Hydrolyzable Phosphorus Species in Environmental Samples
JO  - Analytical chemistry
VL  - 93
IS  - 4
SN  - 1520-6882
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2021-03106
SP  - 2018 - 2025
PY  - 2021
AB  - 31P nuclear magnetic resonance (NMR) spectra can be biased due to the hydrolysis of labile P species during sample treatment and NMR analysis. This paper offers an approach to circumvent this problem by performing sample preparation and analysis in 18O-enriched medium. Heavy 18O isotope atoms were introduced into the resulting artificial hydrolysis products. The NMR signal of 18O-labeled P was shifted upfield relative to the unlabeled P nuclei in natural metabolites. This isotope shift enabled an immediate differentiation of artificial hydrolysis products from natural metabolites. Moreover, the hydrolysis products could be accurately quantified. Our data suggest that the extent to which artificial hydrolysis alters NMR spectra varies among different types of environmental samples. For instance, 72–84% of the detected monoesters in the organic soils of this study were actually artificially hydrolyzed diesters. By contrast, artificial hydrolysis products in the mineral soils used for this study accounted for less than 6% of the total monoesters. Polyphosphate was also hydrolyzed to yield 18O-labeled products in algal biomass.
LB  - PUB:(DE-HGF)16
C6  - 33393290
UR  - <Go to ISI:>//WOS:000618089100020
DO  - DOI:10.1021/acs.analchem.0c03379
UR  - https://juser.fz-juelich.de/record/894217
ER  -