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@ARTICLE{Wang:894217,
      author       = {Wang, Liming and Amelung, Wulf and Willbold, Sabine},
      title        = {18 {O} {I}sotope {L}abeling {C}ombined with 31 {P}
                      {N}uclear {M}agnetic {R}esonance {S}pectroscopy for
                      {A}ccurate {Q}uantification of {H}ydrolyzable {P}hosphorus
                      {S}pecies in {E}nvironmental {S}amples},
      journal      = {Analytical chemistry},
      volume       = {93},
      number       = {4},
      issn         = {1520-6882},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Society},
      reportid     = {FZJ-2021-03106},
      pages        = {2018 - 2025},
      year         = {2021},
      abstract     = {31P nuclear magnetic resonance (NMR) spectra can be biased
                      due to the hydrolysis of labile P species during sample
                      treatment and NMR analysis. This paper offers an approach to
                      circumvent this problem by performing sample preparation and
                      analysis in 18O-enriched medium. Heavy 18O isotope atoms
                      were introduced into the resulting artificial hydrolysis
                      products. The NMR signal of 18O-labeled P was shifted
                      upfield relative to the unlabeled P nuclei in natural
                      metabolites. This isotope shift enabled an immediate
                      differentiation of artificial hydrolysis products from
                      natural metabolites. Moreover, the hydrolysis products could
                      be accurately quantified. Our data suggest that the extent
                      to which artificial hydrolysis alters NMR spectra varies
                      among different types of environmental samples. For
                      instance, $72–84\%$ of the detected monoesters in the
                      organic soils of this study were actually artificially
                      hydrolyzed diesters. By contrast, artificial hydrolysis
                      products in the mineral soils used for this study accounted
                      for less than $6\%$ of the total monoesters. Polyphosphate
                      was also hydrolyzed to yield 18O-labeled products in algal
                      biomass.},
      cin          = {ZEA-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ZEA-3-20090406},
      pnm          = {2151 - Terrestrial ecosystems of the future (POF4-215)},
      pid          = {G:(DE-HGF)POF4-2151},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33393290},
      UT           = {WOS:000618089100020},
      doi          = {10.1021/acs.analchem.0c03379},
      url          = {https://juser.fz-juelich.de/record/894217},
}