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@PHDTHESIS{Zhu:894312,
      author       = {Zhu, Lingfeng},
      title        = {γ-{A}minobutyrate as carbon and nitrogen source for
                      {C}orynebacterium glutamicum and regulation of the catabolic
                      genes by {G}ab{R}},
      volume       = {237},
      school       = {Univ. Düsseldorf},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2021-03172},
      isbn         = {978-3-95806-543-7},
      series       = {Schriften des Forschungszentrums Jülich Reihe
                      Schlüsseltechnologien / Key Technologies},
      pages        = {111},
      year         = {2021},
      note         = {Dissertation, Univ. Düsseldorf, 2020},
      abstract     = {Corynebacterium glutamicum is a Gram-positive soil
                      bacterium widely used in theindustrial production of amino
                      acids such as L-glutamate and L-lysine. C. glutamicumis able
                      to use a variety of carbohydrates, alcohols and organic
                      acids as singlesources of carbon and energy for growth and
                      some also for amino acid production. Inthis thesis, further
                      potential carbon sources were investigated whether they can
                      beused for growth by C. glutamicum. γ-Aminobutyric acid
                      (GABA) is a nonproteinogenicamino acid and widespread in
                      nature from microorganisms to plantsand animals. C.
                      glutamicum showed good growth with GABA as sole carbon
                      andnitrogen source. Remarkably, ammonia and and to a lesser
                      extent urea inhibitedgrowth on GABA, whereas L-glutamine
                      stimulated it. Possible reasons for theseeffects were
                      analyzed. Genome-wide expression analysis revealed that the
                      gabTDPgenes encoding GABA transaminase, succinate
                      semialdehyde dehydrogenase, andGABA permease, respectively,
                      were highly induced in cells grown with GABA ascarbon source
                      compared to glucose-grown cells. The corresponding
                      proteinscatalyze GABA uptake, the transfer of the amino
                      group to 2-oxoglutarate, and theoxidation of the resulting
                      succinate semialdehyde to succinate.
                      Transcriptionalactivation of the gabTDP genes was shown to
                      be strictly dependent on thetranscriptional regulator GabR,
                      which is encoded upstream of and divergent to gabT.A ΔgabR
                      mutant failed to grow on GABA, but not with other carbon
                      sources. Growthof the ΔgabR mutant on GABA could be
                      restored by plasmid-based expression ofeither gabR or
                      gabTDP. Reporter gene studies confirmed that expression of
                      gabTDPis dependent on GabR and GABA. Glucose caused reduced
                      expression of gabTDPpresumably via the cAMP-dependent global
                      regulator GlxR. GabR belongs to thePucR family of
                      transcriptional regulators. Purified GabR eluted as an
                      octamer with anapparent mass of 420 kDa in size-exclusion
                      chromatography and bound specificallyto two binding sites in
                      the gabR-gabT intergenic region extending from -36 to -56
                      andfrom -67 to -87 upstream of the gabT transcriptional
                      start site. These results uncovernew features of
                      actinobacterial GABA utilization.},
      cin          = {IBG-1},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:0001-2021081100},
      url          = {https://juser.fz-juelich.de/record/894312},
}