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@ARTICLE{Ggele:894339,
      author       = {Gögele, Clemens and Konrad, Jens and Hahn, Judith and
                      Breier, Annette and Schröpfer, Michaela and Meyer, Michael
                      and Merkel, Rudolf and Hoffmann, Bernd and Schulze-Tanzil,
                      Gundula},
      title        = {{M}aintenance of {L}igament {H}omeostasis of
                      {S}pheroid-{C}olonized {E}mbroidered and {F}unctionalized
                      {S}caffolds after 3{D} {S}tretch},
      journal      = {International journal of molecular sciences},
      volume       = {22},
      number       = {15},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2021-03187},
      pages        = {8204 -},
      year         = {2021},
      abstract     = {Anterior cruciate ligament (ACL) ruptures are usually
                      treated with autograft implantation to prevent knee
                      instability. Tissue engineered ACL reconstruction is
                      becoming promising to circumvent autograft limitations. The
                      aim was to evaluate the influence of cyclic stretch on
                      lapine (L) ACL fibroblasts on embroidered scaffolds with
                      respect to adhesion, DNA and sulphated glycosaminoglycan
                      (sGAG) contents, gene expression of ligament-associated
                      extracellular matrix genes, such as type I collagen,
                      decorin, tenascin C, tenomodulin, gap junctional connexin 43
                      and the transcription factor Mohawk. Control scaffolds and
                      those functionalized by gas phase fluorination and
                      cross-linked collagen foam were either pre-cultured with a
                      suspension or with spheroids of LACL cells before being
                      subjected to cyclic stretch $(4\%,$ 0.11 Hz, 3 days).
                      Stretch increased significantly the scaffold area colonized
                      with cells but impaired sGAGs and decorin gene expression
                      (functionalized scaffolds seeded with cell suspension).
                      Stretching increased tenascin C, connexin 43 and Mohawk but
                      decreased decorin gene expression (control scaffolds seeded
                      with cell suspension). Pre-cultivation of functionalized
                      scaffolds with spheroids might be the more suitable method
                      for maintaining ligamentogenesis in 3D scaffolds compared to
                      using a cell suspension due to a significantly higher sGAG
                      content in response to stretching and type I collagen gene
                      expression in functionalized scaffolds},
      cin          = {IBI-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-2-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34360970},
      UT           = {WOS:000681896700001},
      doi          = {10.3390/ijms22158204},
      url          = {https://juser.fz-juelich.de/record/894339},
}