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000894458 1001_ $$0P:(DE-Juel1)172901$$aBurmeister, Alina$$b0
000894458 245__ $$a(Optochemical) Control of Synthetic Microbial Coculture Interactions on a Microcolony Level
000894458 260__ $$aWashington, DC$$bACS$$c2021
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000894458 520__ $$aSynthetic microbial cocultures carry enormous potential for applied biotechnology and are increasingly the subject of fundamental research. So far, most cocultures have been designed and characterized based on bulk cultivations without considering the potentially highly heterogeneous and diverse single-cell behavior. However, an in-depth understanding of cocultures including their interacting single cells is indispensable for the development of novel cultivation approaches and control of cocultures. We present the development, validation, and experimental characterization of an optochemically controllable bacterial coculture on a microcolony level consisting of two Corynebacterium glutamicum strains. Our coculture combines an l-lysine auxotrophic strain together with a l-lysine-producing variant carrying the genetically IPTG-mediated induction of l-lysine production. We implemented two control approaches utilizing IPTG as inducer molecule. First, unmodified IPTG was supplemented to the culture enabling a medium-based control of the production of l-lysine, which serves as the main interacting component. Second, optochemical control was successfully performed by utilizing photocaged IPTG activated by appropriate illumination. Both control strategies were validated studying cellular growth on a microcolony level. The novel microfluidic single-cell cultivation strategies applied in this work can serve as a blueprint to validate cellular control strategies of synthetic mono- and cocultures with single-cell resolution at defined environmental conditions.
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000894458 536__ $$0G:(GEPRIS)428038451$$aDFG project 428038451 - SiMBal 2.0: Quantifizierung der Co-Kultur-Leistung und der intrazellulären Interaktionen in Abhängigkeit der Umgebung $$c428038451$$x1
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000894458 7001_ $$0P:(DE-Juel1)176856$$aAkhtar, Qiratt$$b1
000894458 7001_ $$0P:(DE-Juel1)174345$$aHollmann, Lina$$b2
000894458 7001_ $$0P:(DE-Juel1)168172$$aTenhaef, Niklas$$b3
000894458 7001_ $$0P:(DE-Juel1)167181$$aHilgers, Fabienne$$b4
000894458 7001_ $$0P:(DE-Juel1)169329$$aHogenkamp, Fabian$$b5
000894458 7001_ $$0P:(DE-Juel1)138744$$aSokolowsky, Sascha$$b6
000894458 7001_ $$0P:(DE-Juel1)144031$$aMarienhagen, Jan$$b7
000894458 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b8
000894458 7001_ $$0P:(DE-Juel1)140195$$aKohlheyer, Dietrich$$b9
000894458 7001_ $$0P:(DE-Juel1)143612$$aGrünberger, Alexander$$b10$$eCorresponding author
000894458 773__ $$0PERI:(DE-600)2644383-1$$a10.1021/acssynbio.0c00382$$gVol. 10, no. 6, p. 1308 - 1319$$n6$$p1308 - 1319$$tACS synthetic biology$$v10$$x2161-5063$$y2021
000894458 8564_ $$uhttps://juser.fz-juelich.de/record/894458/files/Burmeister_Authorenmanuscript.docx$$yPublished on 2021-06-02. Available in OpenAccess from 2022-06-02.
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