TY  - JOUR
AU  - Burmeister, Alina
AU  - Akhtar, Qiratt
AU  - Hollmann, Lina
AU  - Tenhaef, Niklas
AU  - Hilgers, Fabienne
AU  - Hogenkamp, Fabian
AU  - Sokolowsky, Sascha
AU  - Marienhagen, Jan
AU  - Noack, Stephan
AU  - Kohlheyer, Dietrich
AU  - Grünberger, Alexander
TI  - (Optochemical) Control of Synthetic Microbial Coculture Interactions on a Microcolony Level
JO  - ACS synthetic biology
VL  - 10
IS  - 6
SN  - 2161-5063
CY  - Washington, DC
PB  - ACS
M1  - FZJ-2021-03234
SP  - 1308 - 1319
PY  - 2021
AB  - Synthetic microbial cocultures carry enormous potential for applied biotechnology and are increasingly the subject of fundamental research. So far, most cocultures have been designed and characterized based on bulk cultivations without considering the potentially highly heterogeneous and diverse single-cell behavior. However, an in-depth understanding of cocultures including their interacting single cells is indispensable for the development of novel cultivation approaches and control of cocultures. We present the development, validation, and experimental characterization of an optochemically controllable bacterial coculture on a microcolony level consisting of two Corynebacterium glutamicum strains. Our coculture combines an l-lysine auxotrophic strain together with a l-lysine-producing variant carrying the genetically IPTG-mediated induction of l-lysine production. We implemented two control approaches utilizing IPTG as inducer molecule. First, unmodified IPTG was supplemented to the culture enabling a medium-based control of the production of l-lysine, which serves as the main interacting component. Second, optochemical control was successfully performed by utilizing photocaged IPTG activated by appropriate illumination. Both control strategies were validated studying cellular growth on a microcolony level. The novel microfluidic single-cell cultivation strategies applied in this work can serve as a blueprint to validate cellular control strategies of synthetic mono- and cocultures with single-cell resolution at defined environmental conditions.
LB  - PUB:(DE-HGF)16
C6  - pmid:34075749
UR  - <Go to ISI:>//WOS:000664354700007
DO  - DOI:10.1021/acssynbio.0c00382
UR  - https://juser.fz-juelich.de/record/894458
ER  -