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@ARTICLE{Burmeister:894458,
      author       = {Burmeister, Alina and Akhtar, Qiratt and Hollmann, Lina and
                      Tenhaef, Niklas and Hilgers, Fabienne and Hogenkamp, Fabian
                      and Sokolowsky, Sascha and Marienhagen, Jan and Noack,
                      Stephan and Kohlheyer, Dietrich and Grünberger, Alexander},
      title        = {({O}ptochemical) {C}ontrol of {S}ynthetic {M}icrobial
                      {C}oculture {I}nteractions on a {M}icrocolony {L}evel},
      journal      = {ACS synthetic biology},
      volume       = {10},
      number       = {6},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2021-03234},
      pages        = {1308 - 1319},
      year         = {2021},
      abstract     = {Synthetic microbial cocultures carry enormous potential for
                      applied biotechnology and are increasingly the subject of
                      fundamental research. So far, most cocultures have been
                      designed and characterized based on bulk cultivations
                      without considering the potentially highly heterogeneous and
                      diverse single-cell behavior. However, an in-depth
                      understanding of cocultures including their interacting
                      single cells is indispensable for the development of novel
                      cultivation approaches and control of cocultures. We present
                      the development, validation, and experimental
                      characterization of an optochemically controllable bacterial
                      coculture on a microcolony level consisting of two
                      Corynebacterium glutamicum strains. Our coculture combines
                      an l-lysine auxotrophic strain together with a
                      l-lysine-producing variant carrying the genetically
                      IPTG-mediated induction of l-lysine production. We
                      implemented two control approaches utilizing IPTG as inducer
                      molecule. First, unmodified IPTG was supplemented to the
                      culture enabling a medium-based control of the production of
                      l-lysine, which serves as the main interacting component.
                      Second, optochemical control was successfully performed by
                      utilizing photocaged IPTG activated by appropriate
                      illumination. Both control strategies were validated
                      studying cellular growth on a microcolony level. The novel
                      microfluidic single-cell cultivation strategies applied in
                      this work can serve as a blueprint to validate cellular
                      control strategies of synthetic mono- and cocultures with
                      single-cell resolution at defined environmental conditions.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / DFG project 428038451 - SiMBal
                      2.0: Quantifizierung der Co-Kultur-Leistung und der
                      intrazellulären Interaktionen in Abhängigkeit der
                      Umgebung},
      pid          = {G:(DE-HGF)POF4-2171 / G:(GEPRIS)428038451},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34075749},
      UT           = {WOS:000664354700007},
      doi          = {10.1021/acssynbio.0c00382},
      url          = {https://juser.fz-juelich.de/record/894458},
}