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@ARTICLE{Bitzenhofer:894464,
      author       = {Bitzenhofer, Nora Lisa and Kruse, Luzie and Thies, Stephan
                      and Wynands, Benedikt and Lechtenberg, Thorsten and Rönitz,
                      Jakob and Kozaeva, Ekaterina and Wirth, Nicolas Thilo and
                      Eberlein, Christian and Jaeger, Karl-Erich and Nikel, Pablo
                      Iván and Heipieper, Hermann J. and Wierckx, Nick and
                      Loeschcke, Anita},
      title        = {{T}owards robust {P}seudomonas cell factories to harbour
                      novel biosynthetic pathways},
      journal      = {Essays in biochemistry},
      volume       = {65},
      number       = {2},
      issn         = {1744-1358},
      address      = {London},
      publisher    = {Portland Press},
      reportid     = {FZJ-2021-03240},
      pages        = {319 - 336},
      year         = {2021},
      note         = {Biotechnologie 1},
      abstract     = {Biotechnological production in bacteria enables access to
                      numerous valuable chemical compounds. Nowadays, advanced
                      molecular genetic toolsets, enzyme engineering as well as
                      the combinatorial use of biocatalysts, pathways, and
                      circuits even bring new-to-nature compounds within reach.
                      However, the associated substrates and biosynthetic products
                      often cause severe chemical stress to the bacterial hosts.
                      Species of the Pseudomonas clade thus represent especially
                      valuable chassis as they are endowed with multiple stress
                      response mechanisms, which allow them to cope with a variety
                      of harmful chemicals. A built-in cell envelope stress
                      response enables fast adaptations that sustain membrane
                      integrity under adverse conditions. Further, effective
                      export machineries can prevent intracellular accumulation of
                      diverse harmful compounds. Finally, toxic chemicals such as
                      reactive aldehydes can be eliminated by oxidation and
                      stress-induced damage can be recovered. Exploiting and
                      engineering these features will be essential to support an
                      effective production of natural compounds and new chemicals.
                      In this article, we therefore discuss major resistance
                      strategies of Pseudomonads along with approaches pursued for
                      their targeted exploitation and engineering in a
                      biotechnological context. We further highlight strategies
                      for the identification of yet unknown tolerance-associated
                      genes and their utilisation for engineering next-generation
                      chassis and finally discuss effective measures for pathway
                      fine-tuning to establish stable cell factories for the
                      effective production of natural compounds and novel
                      biochemicals.},
      cin          = {IBG-1 / IMET},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IMET-20090612},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34223620},
      UT           = {WOS:000684146000014},
      doi          = {10.1042/EBC20200173},
      url          = {https://juser.fz-juelich.de/record/894464},
}