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@ARTICLE{Flachbart:894465,
      author       = {Flachbart, Lion Konstantin and Gertzen, Christoph Gerhard
                      Wilhelm and Gohlke, Holger and Marienhagen, Jan},
      title        = {{D}evelopment of a {B}iosensor {P}latform for {P}henolic
                      {C}ompounds {U}sing a {T}ransition {L}igand {S}trategy},
      journal      = {ACS synthetic biology},
      volume       = {10},
      number       = {8},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2021-03241},
      pages        = {2002–2014},
      year         = {2021},
      note         = {Biotechnolgie},
      abstract     = {The time-consuming and laborious characterization of
                      protein or microbial strain designs limits the development
                      of high-performance biocatalysts for biotechnological
                      applications. Here, transcriptional biosensors emerged as
                      valuable tools as they allow for rapid characterization of
                      several thousand variants within a very short time. However,
                      for many molecules of interest, no specific transcriptional
                      regulator determining a biosensor’s specificity is
                      available. We present an approach for rapidly engineering
                      biosensor specificities using a semirational transition
                      ligand approach combined with fluorescence-activated cell
                      sorting. In this two-step approach, a biosensor is first
                      evolved toward a more relaxed-ligand specificity before
                      using the resulting variant as the starting point in a
                      second round of directed evolution toward high specificity
                      for several chemically different ligands. By following this
                      strategy, highly specific biosensors for 4-hydroxybenzoic
                      acid, p-coumaric acid, 5-bromoferulic acid, and 6-methyl
                      salicylic acid were developed, starting from a biosensor for
                      the intracellular detection of trans-cinnamic acid.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / DFG project 417919780 - Zentrum
                      für strukturelle Studien},
      pid          = {G:(DE-HGF)POF4-2171 / G:(GEPRIS)417919780},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34369151},
      UT           = {WOS:000687920000019},
      doi          = {10.1021/acssynbio.1c00165},
      url          = {https://juser.fz-juelich.de/record/894465},
}