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| 001 | 894465 | ||
| 005 | 20230815122845.0 | ||
| 024 | 7 | _ | |a 10.1021/acssynbio.1c00165 |2 doi |
| 024 | 7 | _ | |a 2128/28567 |2 Handle |
| 024 | 7 | _ | |a altmetric:111534990 |2 altmetric |
| 024 | 7 | _ | |a 34369151 |2 pmid |
| 024 | 7 | _ | |a WOS:000687920000019 |2 WOS |
| 037 | _ | _ | |a FZJ-2021-03241 |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Flachbart, Lion Konstantin |0 P:(DE-Juel1)162145 |b 0 |
| 245 | _ | _ | |a Development of a Biosensor Platform for Phenolic Compounds Using a Transition Ligand Strategy |
| 260 | _ | _ | |a Washington, DC |c 2021 |b ACS |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1641298266_23856 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a Biotechnolgie |
| 520 | _ | _ | |a The time-consuming and laborious characterization of protein or microbial strain designs limits the development of high-performance biocatalysts for biotechnological applications. Here, transcriptional biosensors emerged as valuable tools as they allow for rapid characterization of several thousand variants within a very short time. However, for many molecules of interest, no specific transcriptional regulator determining a biosensor’s specificity is available. We present an approach for rapidly engineering biosensor specificities using a semirational transition ligand approach combined with fluorescence-activated cell sorting. In this two-step approach, a biosensor is first evolved toward a more relaxed-ligand specificity before using the resulting variant as the starting point in a second round of directed evolution toward high specificity for several chemically different ligands. By following this strategy, highly specific biosensors for 4-hydroxybenzoic acid, p-coumaric acid, 5-bromoferulic acid, and 6-methyl salicylic acid were developed, starting from a biosensor for the intracellular detection of trans-cinnamic acid. |
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| 536 | _ | _ | |a DFG project 417919780 - Zentrum für strukturelle Studien |0 G:(GEPRIS)417919780 |c 417919780 |x 1 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Gertzen, Christoph Gerhard Wilhelm |0 P:(DE-Juel1)174133 |b 1 |
| 700 | 1 | _ | |a Gohlke, Holger |0 P:(DE-Juel1)172663 |b 2 |
| 700 | 1 | _ | |a Marienhagen, Jan |0 P:(DE-Juel1)144031 |b 3 |e Corresponding author |
| 773 | _ | _ | |a 10.1021/acssynbio.1c00165 |g p. acssynbio.1c00165 |0 PERI:(DE-600)2644383-1 |n 8 |p 2002–2014 |t ACS synthetic biology |v 10 |y 2021 |x 2161-5063 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/894465/files/Invoice_APC600235924.pdf |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/894465/files/acssynbio.1c00165.pdf |y OpenAccess |
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| 914 | 1 | _ | |y 2021 |
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