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@ARTICLE{Kamel:894605,
      author       = {Kamel, Michael and Löwe, Maryna and Schott-Verdugo,
                      Stephan and Gohlke, Holger and Kedrov, Alexej},
      title        = {{U}nsaturated fatty acids augment protein transport via the
                      {S}ec{A}:{S}ec{YEG} translocon},
      journal      = {The FEBS journal},
      volume       = {289},
      number       = {1},
      issn         = {1742-4658},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2021-03306},
      pages        = {140-162},
      year         = {2022},
      abstract     = {The translocon SecYEG and the associated ATPase SecA form
                      the primary protein secretion system in the cytoplasmic
                      membrane of bacteria. The secretion is essentially dependent
                      on the surrounding lipids, but the mechanistic understanding
                      of their role in SecA : SecYEG activity is sparse. Here, we
                      reveal that the unsaturated fatty acids (UFAs) of the
                      membrane phospholipids, including tetraoleoyl-cardiolipin,
                      stimulate SecA : SecYEG-mediated protein translocation up to
                      ten-fold. Biophysical analysis and molecular dynamics
                      simulations show that UFAs increase the area per lipid and
                      cause loose packing of lipid head groups, where the
                      N-terminal amphipathic helix of SecA docks. While UFAs do
                      not affect the translocon folding, they promote SecA binding
                      to the membrane, and the effect is enhanced up to fivefold
                      at elevated ionic strength. Tight SecA : lipid interactions
                      convert into the augmented translocation. Our results
                      identify the fatty acid structure as a notable factor in
                      SecA : SecYEG activity, which may be crucial for protein
                      secretion in bacteria, which actively change their membrane
                      composition in response to their habitat.},
      cin          = {JSC / NIC / IBI-7 / IBG-4},
      ddc          = {610},
      cid          = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406 /
                      I:(DE-Juel1)IBI-7-20200312 / I:(DE-Juel1)IBG-4-20200403},
      pnm          = {5111 - Domain-Specific Simulation $\&$ Data Life Cycle Labs
                      (SDLs) and Research Groups (POF4-511) / 2171 - Biological
                      and environmental resources for sustainable use (POF4-217) /
                      2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217) /
                      Forschergruppe Gohlke $(hkf7_20200501)$ / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5111 / G:(DE-HGF)POF4-2171 /
                      G:(DE-HGF)POF4-2172 / $G:(DE-Juel1)hkf7_20200501$ /
                      G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34312977},
      UT           = {WOS:000682136100001},
      doi          = {10.1111/febs.16140},
      url          = {https://juser.fz-juelich.de/record/894605},
}