000894706 001__ 894706
000894706 005__ 20210930133542.0
000894706 0247_ $$2doi$$a10.1073/pnas.2101526118
000894706 0247_ $$2ISSN$$a0027-8424
000894706 0247_ $$2ISSN$$a1091-6490
000894706 0247_ $$2Handle$$a2128/28637
000894706 0247_ $$2altmetric$$aaltmetric:112426579
000894706 0247_ $$2pmid$$apmid:34446550
000894706 0247_ $$2WOS$$aWOS:000691662100011
000894706 037__ $$aFZJ-2021-03361
000894706 041__ $$aEnglish
000894706 082__ $$a500
000894706 1001_ $$0P:(DE-HGF)0$$aKirschner, Gwendolyn K.$$b0
000894706 245__ $$aENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF–containing protein that controls root growth angle in barley and wheat
000894706 260__ $$aWashington, DC$$bNational Acad. of Sciences$$c2021
000894706 3367_ $$2DRIVER$$aarticle
000894706 3367_ $$2DataCite$$aOutput Types/Journal article
000894706 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1631612041_21944
000894706 3367_ $$2BibTeX$$aARTICLE
000894706 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000894706 3367_ $$00$$2EndNote$$aJournal Article
000894706 520__ $$aThe root growth angle defines how roots grow toward the gravity vector and is among the most important determinants of root system architecture. It controls water uptake capacity, nutrient use efficiency, stress resilience, and, as a consequence, yield of crop plants. We demonstrated that the egt2 (enhanced gravitropism 2) mutant of barley exhibits steeper root growth of seminal and lateral roots and an auxin-independent higher responsiveness to gravity compared to wild-type plants. We cloned the EGT2 gene by a combination of bulked-segregant analysis and whole genome sequencing. Subsequent validation experiments by an independent CRISPR/Cas9 mutant allele demonstrated that egt2 encodes a STERILE ALPHA MOTIF domain–containing protein. In situ hybridization experiments illustrated that EGT2 is expressed from the root cap to the elongation zone. We demonstrated the evolutionary conserved role of EGT2 in root growth angle control between barley and wheat by knocking out the EGT2 orthologs in the A and B genomes of tetraploid durum wheat. By combining laser capture microdissection with RNA sequencing, we observed that seven expansin genes were transcriptionally down-regulated in the elongation zone. This is consistent with a role of EGT2 in this region of the root where the effect of gravity sensing is executed by differential cell elongation. Our findings suggest that EGT2 is an evolutionary conserved regulator of root growth angle in barley and wheat that could be a valuable target for root-based crop improvement strategies in cereals
000894706 536__ $$0G:(DE-HGF)POF4-2171$$a2171 - Biological and environmental resources for sustainable use (POF4-217)$$cPOF4-217$$fPOF IV$$x0
000894706 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
000894706 7001_ $$00000-0002-4319-8761$$aRosignoli, Serena$$b1
000894706 7001_ $$00000-0003-0406-6966$$aGuo, Li$$b2
000894706 7001_ $$00000-0002-3642-9381$$aVardanega, Isaia$$b3
000894706 7001_ $$00000-0001-8265-1656$$aImani, Jafargholi$$b4
000894706 7001_ $$0P:(DE-HGF)0$$aAltmüller, Janine$$b5
000894706 7001_ $$0P:(DE-HGF)0$$aMilner, Sara G.$$b6
000894706 7001_ $$00000-0001-7405-4456$$aBalzano, Raffaella$$b7
000894706 7001_ $$0P:(DE-Juel1)129373$$aNagel, Kerstin A.$$b8
000894706 7001_ $$0P:(DE-Juel1)131784$$aPflugfelder, Daniel$$b9
000894706 7001_ $$00000-0002-1712-825X$$aForestan, Cristian$$b10
000894706 7001_ $$00000-0001-6170-4587$$aBovina, Riccardo$$b11
000894706 7001_ $$0P:(DE-Juel1)165733$$aKoller, Robert$$b12
000894706 7001_ $$00000-0001-7184-9472$$aStöcker, Tyll G.$$b13
000894706 7001_ $$0P:(DE-HGF)0$$aMascher, Martin$$b14
000894706 7001_ $$0P:(DE-HGF)0$$aSimmonds, James$$b15
000894706 7001_ $$00000-0002-9814-1770$$aUauy, Cristobal$$b16
000894706 7001_ $$00000-0002-1527-3752$$aSchoof, Heiko$$b17
000894706 7001_ $$00000-0001-9143-9569$$aTuberosa, Roberto$$b18
000894706 7001_ $$00000-0002-0338-8894$$aSalvi, Silvio$$b19$$eCorresponding author
000894706 7001_ $$0P:(DE-HGF)0$$aHochholdinger, Frank$$b20$$eCorresponding author
000894706 773__ $$0PERI:(DE-600)1461794-8$$a10.1073/pnas.2101526118$$gVol. 118, no. 35, p. e2101526118 -$$n35$$pe2101526118 -$$tProceedings of the National Academy of Sciences of the United States of America$$v118$$x1091-6490$$y2021
000894706 8564_ $$uhttps://juser.fz-juelich.de/record/894706/files/Kirschner_PNAS_2021.pdf$$yOpenAccess
000894706 8564_ $$uhttps://juser.fz-juelich.de/record/894706/files/Supplements.pdf$$yOpenAccess
000894706 8564_ $$uhttps://juser.fz-juelich.de/record/894706/files/e2101526118.full.pdf$$yOpenAccess
000894706 909CO $$ooai:juser.fz-juelich.de:894706$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000894706 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129373$$aForschungszentrum Jülich$$b8$$kFZJ
000894706 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131784$$aForschungszentrum Jülich$$b9$$kFZJ
000894706 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165733$$aForschungszentrum Jülich$$b12$$kFZJ
000894706 9131_ $$0G:(DE-HGF)POF4-217$$1G:(DE-HGF)POF4-210$$2G:(DE-HGF)POF4-200$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-2171$$aDE-HGF$$bForschungsbereich Erde und Umwelt$$lErde im Wandel – Unsere Zukunft nachhaltig gestalten$$vFür eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten$$x0
000894706 9141_ $$y2021
000894706 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bP NATL ACAD SCI USA : 2019$$d2021-01-29
000894706 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000894706 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bP NATL ACAD SCI USA : 2019$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000894706 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0430$$2StatID$$aNational-Konsortium$$d2021-01-29$$wger
000894706 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2021-01-29
000894706 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-01-29
000894706 920__ $$lyes
000894706 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$kIBG-2$$lPflanzenwissenschaften$$x0
000894706 980__ $$ajournal
000894706 980__ $$aVDB
000894706 980__ $$aUNRESTRICTED
000894706 980__ $$aI:(DE-Juel1)IBG-2-20101118
000894706 9801_ $$aFullTexts