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@ARTICLE{Lvenich:894715,
author = {Lövenich, Lukas and Dreissen, Georg and Hoffmann,
Christina and Konrad, Jens and Springer, Ronald and
Höhfeld, Jörg and Merkel, Rudolf and Hoffmann, Bernd},
title = {{S}train induced mechanoresponse depends on cell
contractility and {BAG}3-mediated autophagy},
journal = {Molecular biology of the cell},
volume = {32},
number = {20},
issn = {1939-4586},
address = {Bethesda, Md.},
publisher = {American Society for Cell Biology},
reportid = {FZJ-2021-03367},
pages = {},
year = {2021},
abstract = {Basically, all mammalian tissues are constantly exposed to
a variety of environmental mechanical signals. Depending on
the signal strength, mechanics intervenes in a multitude of
cellular processes and is thus capable of inducing simple
cellular adaptations but also complex differentiation
processes and even apoptosis. The underlying recognition
typically depends on mechanosensitive proteins, which most
often sense the mechanical signal for the induction of a
cellular signaling cascade by changing their protein
conformation. However, the fate of mechanosensors after
mechanical stress application is still poorly understood,
and it remains unclear whether protein degradation pathways
affect the mechanosensitivity of cells. Here, we show that
cyclic stretch induces autophagosome formation in a
time-dependent manner. Formation depends on the cochaperone
BAG family molecular chaperone regulator 3 (BAG3) and thus
likely involves BAG3-mediated chaperone-assisted selective
autophagy. Furthermore, we demonstrate that strain-induced
cell reorientation is clearly delayed upon inhibition of
autophagy, suggesting a bidirectional cross-talk between
mechanotransduction and autophagic degradation. The strength
of the observed delay depends on stable adhesion structures
and stress fiber formation in a Ras homologue family member
A (RhoA)-dependent manner.},
cin = {IBI-2},
ddc = {570},
cid = {I:(DE-Juel1)IBI-2-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524) / DFG project 388932620 - FOR 2743: Zelluläre
Schutzmechanismen gegen mechanischen Stress},
pid = {G:(DE-HGF)POF4-5241 / G:(GEPRIS)388932620},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34379447},
UT = {WOS:000743181900005},
doi = {10.1091/mbc.E21-05-0254},
url = {https://juser.fz-juelich.de/record/894715},
}