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000894762 0247_ $$2doi$$a10.1101/2021.02.12.430943
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000894762 037__ $$aFZJ-2021-03376
000894762 1001_ $$0P:(DE-Juel1)169979$$aLabib, Mohamed$$b0
000894762 245__ $$aMetabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum
000894762 260__ $$c2021
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000894762 520__ $$a3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of interest in the chemical industry as a building block for novel polymers and has wide potential for pharmaceutical applications due to its antioxidant, anti-inflammatory, and antiviral properties. In the present study, we designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d -xylose for microbial production of PCA. The engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L −1 ) from d -xylose as the primary carbon and energy source. The corresponding yield was , which corresponds to 38 % of the maximum theoretical yield and is 14-fold higher compared to the parental producer strain on d -glucose. By establishing a one-pot bioreactor cultivation process followed by subsequent process optimization, the same maximum titer and a total amount of 16.5 ± 1.1 g was reached. Downstream processing of PCA from this fermentation broth was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. Since PCA turned out to be electrochemically unstable in combination with several anode materials, a threechamber electrolysis setup was established to crystallize PCA and to avoid direct anode contact. This resulted in a maximum final purity of 95.4 %. In summary, the established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks.
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000894762 7001_ $$0P:(DE-HGF)0$$aGörtz, Jonas$$b1
000894762 7001_ $$0P:(DE-Juel1)166290$$aBrüsseler, Christian$$b2
000894762 7001_ $$0P:(DE-Juel1)157678$$aKallscheuer, Nicolai$$b3
000894762 7001_ $$0P:(DE-Juel1)129023$$aGätgens, Jochem$$b4
000894762 7001_ $$00000-0001-6551-5695$$aJupke, Andreas$$b5
000894762 7001_ $$0P:(DE-Juel1)144031$$aMarienhagen, Jan$$b6
000894762 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b7$$eCorresponding author
000894762 773__ $$a10.1101/2021.02.12.430943
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