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@ARTICLE{Labib:894762,
      author       = {Labib, Mohamed and Görtz, Jonas and Brüsseler, Christian
                      and Kallscheuer, Nicolai and Gätgens, Jochem and Jupke,
                      Andreas and Marienhagen, Jan and Noack, Stephan},
      title        = {{M}etabolic and {P}rocess {E}ngineering for {M}icrobial
                      {P}roduction of {P}rotocatechuate from {X}ylose with
                      {C}orynebacterium glutamicum},
      reportid     = {FZJ-2021-03376},
      year         = {2021},
      abstract     = {3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic
                      compound naturally found in edible vegetables and medicinal
                      herbs. PCA is of interest in the chemical industry as a
                      building block for novel polymers and has wide potential for
                      pharmaceutical applications due to its antioxidant,
                      anti-inflammatory, and antiviral properties. In the present
                      study, we designed and constructed a novel Corynebacterium
                      glutamicum strain to enable the efficient utilization of d
                      -xylose for microbial production of PCA. The engineered
                      strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ±
                      1.9 g L −1 ) from d -xylose as the primary carbon and
                      energy source. The corresponding yield was , which
                      corresponds to 38 $\%$ of the maximum theoretical yield and
                      is 14-fold higher compared to the parental producer strain
                      on d -glucose. By establishing a one-pot bioreactor
                      cultivation process followed by subsequent process
                      optimization, the same maximum titer and a total amount of
                      16.5 ± 1.1 g was reached. Downstream processing of PCA from
                      this fermentation broth was realized via electrochemically
                      induced crystallization by taking advantage of the
                      pH-dependent properties of PCA. Since PCA turned out to be
                      electrochemically unstable in combination with several anode
                      materials, a threechamber electrolysis setup was established
                      to crystallize PCA and to avoid direct anode contact. This
                      resulted in a maximum final purity of 95.4 $\%.$ In summary,
                      the established PCA production process represents a highly
                      sustainable approach, which will serve as a blueprint for
                      the bio-based production of other hydroxybenzoic acids from
                      alternative sugar feedstocks.},
      cin          = {IBG-1},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)25},
      doi          = {10.1101/2021.02.12.430943},
      url          = {https://juser.fz-juelich.de/record/894762},
}