guest ::
| Home > Publications database > Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum > print |
| Home > Publications database > Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum > print |
| 001 | 894762 | ||
| 005 | 20210912010929.0 | ||
| 024 | 7 | _ | |a 10.1101/2021.02.12.430943 |2 doi |
| 024 | 7 | _ | |a 2128/28599 |2 Handle |
| 024 | 7 | _ | |a altmetric:100139792 |2 altmetric |
| 037 | _ | _ | |a FZJ-2021-03376 |
| 100 | 1 | _ | |a Labib, Mohamed |0 P:(DE-Juel1)169979 |b 0 |
| 245 | _ | _ | |a Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum |
| 260 | _ | _ | |c 2021 |
| 336 | 7 | _ | |a Preprint |b preprint |m preprint |0 PUB:(DE-HGF)25 |s 1631004476_12436 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a WORKING_PAPER |2 ORCID |
| 336 | 7 | _ | |a Electronic Article |0 28 |2 EndNote |
| 336 | 7 | _ | |a preprint |2 DRIVER |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Working Paper |2 DataCite |
| 520 | _ | _ | |a 3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of interest in the chemical industry as a building block for novel polymers and has wide potential for pharmaceutical applications due to its antioxidant, anti-inflammatory, and antiviral properties. In the present study, we designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d -xylose for microbial production of PCA. The engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L −1 ) from d -xylose as the primary carbon and energy source. The corresponding yield was , which corresponds to 38 % of the maximum theoretical yield and is 14-fold higher compared to the parental producer strain on d -glucose. By establishing a one-pot bioreactor cultivation process followed by subsequent process optimization, the same maximum titer and a total amount of 16.5 ± 1.1 g was reached. Downstream processing of PCA from this fermentation broth was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. Since PCA turned out to be electrochemically unstable in combination with several anode materials, a threechamber electrolysis setup was established to crystallize PCA and to avoid direct anode contact. This resulted in a maximum final purity of 95.4 %. In summary, the established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks. |
| 536 | _ | _ | |a 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) |0 G:(DE-HGF)POF4-2172 |c POF4-217 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Görtz, Jonas |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Brüsseler, Christian |0 P:(DE-Juel1)166290 |b 2 |
| 700 | 1 | _ | |a Kallscheuer, Nicolai |0 P:(DE-Juel1)157678 |b 3 |
| 700 | 1 | _ | |a Gätgens, Jochem |0 P:(DE-Juel1)129023 |b 4 |
| 700 | 1 | _ | |a Jupke, Andreas |0 0000-0001-6551-5695 |b 5 |
| 700 | 1 | _ | |a Marienhagen, Jan |0 P:(DE-Juel1)144031 |b 6 |
| 700 | 1 | _ | |a Noack, Stephan |0 P:(DE-Juel1)129050 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1101/2021.02.12.430943 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/894762/files/2021.02.12.430943.full.pdf |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:894762 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)169979 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)129023 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 6 |6 P:(DE-Juel1)144031 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 7 |6 P:(DE-Juel1)129050 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Erde und Umwelt |l Erde im Wandel – Unsere Zukunft nachhaltig gestalten |1 G:(DE-HGF)POF4-210 |0 G:(DE-HGF)POF4-217 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-200 |4 G:(DE-HGF)POF |v Für eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten |9 G:(DE-HGF)POF4-2172 |x 0 |
| 914 | 1 | _ | |y 2021 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBG-1-20101118 |k IBG-1 |l Biotechnologie |x 0 |
| 980 | 1 | _ | |a FullTexts |
| 980 | _ | _ | |a preprint |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|