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@ARTICLE{Zhang:894822,
      author       = {Zhang, Yue and Lu, Peng-Han and Rotunno, Enzo and Troiani,
                      Filippo and van Schayck, J. Paul and Tavabi, Amir H. and
                      Dunin-Borkowski, Rafal E. and Grillo, Vincenzo and Peters,
                      Peter J. and Ravelli, Raimond B. G.},
      title        = {{S}ingle-particle cryo-{EM}: alternative schemes to improve
                      dose efficiency},
      journal      = {Journal of synchrotron radiation},
      volume       = {28},
      number       = {5},
      issn         = {1600-5775},
      address      = {[S.l.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2021-03408},
      pages        = {1343 - 1356},
      year         = {2021},
      abstract     = {Imaging of biomolecules by ionizing radiation, such as
                      electrons, causes radiation damage which introduces
                      structural and compositional changes of the specimen. The
                      total number of high-energy electrons per surface area that
                      can be used for imaging in cryogenic electron microscopy
                      (cryo-EM) is severely restricted due to radiation damage,
                      resulting in low signal-to-noise ratios (SNR). High
                      resolution details are dampened by the transfer function of
                      the microscope and detector, and are the first to be lost as
                      radiation damage alters the individual molecules which are
                      presumed to be identical during averaging. As a consequence,
                      radiation damage puts a limit on the particle size and
                      sample heterogeneity with which electron microscopy (EM) can
                      deal. Since a transmission EM (TEM) image is formed from the
                      scattering process of the electron by the specimen
                      interaction potential, radiation damage is inevitable.
                      However, we can aim to maximize the information transfer for
                      a given dose and increase the SNR by finding alternatives to
                      the conventional phase-contrast cryo-EM techniques. Here
                      some alternative transmission electron microscopy techniques
                      are reviewed, including phase plate, multi-pass transmission
                      electron microscopy, off-axis holography, ptychography and a
                      quantum sorter. Their prospects for providing more or
                      complementary structural information within the limited
                      lifetime of the sample are discussed.},
      cin          = {ER-C-1},
      ddc          = {550},
      cid          = {I:(DE-Juel1)ER-C-1-20170209},
      pnm          = {5351 - Platform for Correlative, In Situ and Operando
                      Characterization (POF4-535) / Q-SORT - QUANTUM SORTER
                      (766970)},
      pid          = {G:(DE-HGF)POF4-5351 / G:(EU-Grant)766970},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34475283},
      UT           = {WOS:000693111600009},
      doi          = {10.1107/S1600577521007931},
      url          = {https://juser.fz-juelich.de/record/894822},
}