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@ARTICLE{Harris:894854,
      author       = {Harris, Andrzej and Wagner, Manuel and Du, Dijun and
                      Raschka, Stefanie and Nentwig, Lea-Marie and Gohlke, Holger
                      and Smits, Sander H. J. and Luisi, Ben F. and Schmitt, Lutz},
      title        = {{S}tructure and efflux mechanism of the yeast pleiotropic
                      drug resistance transporter {P}dr5},
      journal      = {Nature Communications},
      volume       = {12},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {FZJ-2021-03425},
      pages        = {5254},
      year         = {2021},
      abstract     = {Pdr5, a member of the extensive ABC transporter
                      superfamily, is representative of a clinically relevant
                      subgroup involved in pleiotropic drug resistance. Pdr5 and
                      its homologues drive drug efflux through uncoupled
                      hydrolysis of nucleotides, enabling organisms such as
                      baker’s yeast and pathogenic fungi to survive in the
                      presence of chemically diverse antifungal agents. Here, we
                      present the molecular structure of Pdr5 solved with single
                      particle cryo-EM, revealing details of an ATP-driven
                      conformational cycle, which mechanically drives drug
                      translocation through an amphipathic channel, and a clamping
                      switch within a conserved linker loop that acts as a
                      nucleotide sensor. One half of the transporter remains
                      nearly invariant throughout the cycle, while its partner
                      undergoes changes that are transmitted across inter-domain
                      interfaces to support a peristaltic motion of the pumped
                      molecule. The efflux model proposed here rationalises the
                      pleiotropic impact of Pdr5 and opens new avenues for the
                      development of effective antifungal compounds.},
      cin          = {JSC / NIC / IBI-7 / IBG-4},
      ddc          = {500},
      cid          = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406 /
                      I:(DE-Juel1)IBI-7-20200312 / I:(DE-Juel1)IBG-4-20200403},
      pnm          = {5111 - Domain-Specific Simulation Data Life Cycle Labs
                      (SDLs) and Research Groups (POF4-511) / 2171 - Biological
                      and environmental resources for sustainable use (POF4-217) /
                      2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217) /
                      Forschergruppe Gohlke $(hkf7_20200501)$ / DFG project
                      417919780 - Zentrum für strukturelle Studien},
      pid          = {G:(DE-HGF)POF4-5111 / G:(DE-HGF)POF4-2171 /
                      G:(DE-HGF)POF4-2172 / $G:(DE-Juel1)hkf7_20200501$ /
                      G:(GEPRIS)417919780},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34489436},
      UT           = {WOS:000694666900027},
      doi          = {10.1038/s41467-021-25574-8},
      url          = {https://juser.fz-juelich.de/record/894854},
}