TY  - JOUR
AU  - Schütz, Markus B.
AU  - Renner, Alexander M.
AU  - Ilyas, Shaista
AU  - Lê, Khan
AU  - Guliyev, Mehrab
AU  - Krapf, Philipp
AU  - Neumaier, Bernd
AU  - Mathur, Sanjay
TI  - 18F-Labeled magnetic nanovectors for bimodal cellular imaging
JO  - Biomaterials science
VL  - 9
IS  - 13
SN  - 2047-4849
CY  - Cambridge
PB  - Royal Soc. of Chemistry
M1  - FZJ-2021-03505
SP  - 4717 - 4727
PY  - 2021
AB  - Surface modification of nanocarriers enables selective attachment to specific molecular targets within a complex biological environment. Besides the enhanced uptake due to specific interactions, the surfaceligands can be utilized for radiolabeling applications for bimodal imaging ensured by positron emission topography (PET) and magnetic resonance imaging (MRI) functions in one source. Herein, we describethe surface functionalization of magnetite (Fe3O4) with folic acid as a target vector. Additionally, the magnetic nanocarriers were conjugated with appropriate ligands for subsequent copper-catalyzed azide–alkyne cycloaddition or carbodiimide coupling reactions to successfully achieve radiolabeling with the PET-emitter 18F. The phase composition (XRD) and size analysis (TEM) confirmed the formation of Fe3O4nanoparticles (6.82 nm ± 0.52 nm). The quantification of various surface functionalities was performed by Fourier-transform infrared spectroscopy (FT-IR) and ultraviolet-visible microscopy (UV-Vis). An innovativemagnetic-HPLC method was developed in this work for the determination of the radiochemical yield of the 18F-labeled NPs. The as-prepared Fe3O4 particles demonstrated high radiochemical yields andshowed high cellular uptake in a folate receptor overexpressing MCF-7 cell line, validating bimodalimaging chemical design and a magnetic HPLC system. This novel approach, combining folic acidcappedFe3O4 nanocarriers as a targeting vector with 18F labeling, is promising to apply this probe forbimodal PET/MR-studies.
LB  - PUB:(DE-HGF)16
C6  - pmid:34032225
UR  - <Go to ISI:>//WOS:000653661200001
DO  - DOI:10.1039/D1BM00616A
UR  - https://juser.fz-juelich.de/record/894976
ER  -