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@ARTICLE{Loogen:896722,
      author       = {Loogen, Judith and Müller, Andreas and Balzer, Arne and
                      Weber, Sophie and Schmitz, Kathrin and Krug, Roxanne and
                      Schaffrath, Ulrich and Pietruszka, Jörg and Conrath, Uwe
                      and Büchs, Jochen},
      title        = {{A}n illuminated respiratory activity monitoring system
                      identifies priming-active compounds in plant seedlings-1},
      journal      = {BMC plant biology},
      volume       = {21},
      number       = {1},
      issn         = {1471-2229},
      address      = {London},
      publisher    = {BioMed Central},
      reportid     = {FZJ-2021-03555},
      pages        = {324},
      year         = {2021},
      abstract     = {BackgroundGrowing large crop monocultures and heavily using
                      pesticides enhances the evolution of pesticide-insensitive
                      pests and pathogens. To reduce pesticide use in crop
                      cultivation, the application of priming-active compounds
                      (PrimACs) is a welcome alternative. PrimACs strengthen the
                      plant immune system and could thus help to protect plants
                      with lower amounts of pesticides. PrimACs can be identified,
                      for example, by their capacity to enhance the respiratory
                      activity of parsley cells in culture as determined by the
                      oxygen transfer rate (OTR) using the respiration activity
                      monitoring system (RAMOS) or its miniaturized version,
                      µRAMOS. The latter was designed for with suspensions of
                      bacteria and yeast cells in microtiter plates (MTPs). So
                      far, RAMOS or µRAMOS have not been applied to adult plants
                      or seedlings, which would overcome the limitation of
                      (µ)RAMOS to plant suspension cell cultures.ResultsIn this
                      work, we introduce a modified µRAMOS for analysis of plant
                      seedlings. The novel device allows illuminating the
                      seedlings and records the respiratory activity in each well
                      of a 48-well MTP. To validate the suitability of the setup
                      for identifying novel PrimAC in Arabidopsis thaliana,
                      seedlings were grown in MTP for seven days and treated with
                      the known PrimAC salicylic acid (SA; positive control) and
                      the PrimAC candidate methyl
                      1-(3,4-dihydroxyphenyl)-2-oxocyclopentane-1-carboxylate
                      (Tyr020). Twenty-eight h after treatment, the seedlings were
                      elicited with flg22, a 22-amino acid peptide of bacterial
                      flagellin. Upon elicitation, the respiratory activity was
                      monitored. The evaluation of the OTR course reveals Tyr020
                      as a likely PrimAC. The priming-inducing activity of Tyr020
                      was confirmed using molecular biological analyses in A.
                      thaliana seedlings.ConclusionWe disclose the suitability of
                      µRAMOS for identifying PrimACs in plant seedlings. The
                      difference in OTR during a night period between primed and
                      unprimed plants was distinguishable after elicitation with
                      flg22. Thus, it has been shown that the µRAMOS device can
                      be used for a reliable screening for PrimACs in plant
                      seedlings.},
      cin          = {IBOC / IBG-1 / IBG-2},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118 /
                      I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34225655},
      UT           = {WOS:000672470400003},
      doi          = {10.1186/s12870-021-03100-8},
      url          = {https://juser.fz-juelich.de/record/896722},
}