%0 Journal Article
%A Labib, Mohamed
%A Görtz, Jonas
%A Brüsseler, Christian
%A Kallscheuer, Nicolai
%A Gätgens, Jochem
%A Jupke, Andreas
%A Marienhagen, Jan
%A Noack, Stephan
%T Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum
%J Biotechnology & bioengineering
%V 118
%N 11
%@ 1097-0290
%C New York, NY [u.a.]
%I Wiley
%M FZJ-2021-03712
%P bit.27909
%D 2021
%X 3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of high interest in the chemical industry and has wide potential for pharmaceutical applications. We designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d-xylose for microbial production of PCA. Shake flask cultivation of the engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from d-xylose as the primary carbon and energy source. The corresponding yield was 0.33 C-mol PCA per C-mol d-xylose, which corresponds to 38% of the maximum theoretical yield. Under growth-decoupled bioreactor conditions, a comparable PCA titer and a total amount of 16.5 ± 1.1 g PCA could be achieved when d-glucose and d-xylose were combined as orthogonal carbon substrates for biocatalyst provision and product synthesis, respectively. Downstream processing of PCA was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. This resulted in a maximum final purity of 95.4%. The established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 34343343
%U <Go to ISI:>//WOS:000686279100001
%R 10.1002/bit.27909
%U https://juser.fz-juelich.de/record/897250