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000897250 1001_ $$0P:(DE-Juel1)169979$$aLabib, Mohamed$$b0
000897250 245__ $$aMetabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum
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000897250 520__ $$a3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of high interest in the chemical industry and has wide potential for pharmaceutical applications. We designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d-xylose for microbial production of PCA. Shake flask cultivation of the engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from d-xylose as the primary carbon and energy source. The corresponding yield was 0.33 C-mol PCA per C-mol d-xylose, which corresponds to 38% of the maximum theoretical yield. Under growth-decoupled bioreactor conditions, a comparable PCA titer and a total amount of 16.5 ± 1.1 g PCA could be achieved when d-glucose and d-xylose were combined as orthogonal carbon substrates for biocatalyst provision and product synthesis, respectively. Downstream processing of PCA was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. This resulted in a maximum final purity of 95.4%. The established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks.
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000897250 7001_ $$0P:(DE-HGF)0$$aGörtz, Jonas$$b1
000897250 7001_ $$0P:(DE-Juel1)166290$$aBrüsseler, Christian$$b2
000897250 7001_ $$0P:(DE-Juel1)157678$$aKallscheuer, Nicolai$$b3
000897250 7001_ $$0P:(DE-Juel1)129023$$aGätgens, Jochem$$b4$$ufzj
000897250 7001_ $$00000-0001-6551-5695$$aJupke, Andreas$$b5
000897250 7001_ $$0P:(DE-Juel1)144031$$aMarienhagen, Jan$$b6
000897250 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b7$$eCorresponding author
000897250 773__ $$0PERI:(DE-600)1480809-2$$a10.1002/bit.27909$$gp. bit.27909$$n11$$pbit.27909$$tBiotechnology & bioengineering$$v118$$x1097-0290$$y2021
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