TY  - JOUR
AU  - Labib, Mohamed
AU  - Görtz, Jonas
AU  - Brüsseler, Christian
AU  - Kallscheuer, Nicolai
AU  - Gätgens, Jochem
AU  - Jupke, Andreas
AU  - Marienhagen, Jan
AU  - Noack, Stephan
TI  - Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum
JO  - Biotechnology & bioengineering
VL  - 118
IS  - 11
SN  - 1097-0290
CY  - New York, NY [u.a.]
PB  - Wiley
M1  - FZJ-2021-03712
SP  - bit.27909
PY  - 2021
AB  - 3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of high interest in the chemical industry and has wide potential for pharmaceutical applications. We designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d-xylose for microbial production of PCA. Shake flask cultivation of the engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from d-xylose as the primary carbon and energy source. The corresponding yield was 0.33 C-mol PCA per C-mol d-xylose, which corresponds to 38% of the maximum theoretical yield. Under growth-decoupled bioreactor conditions, a comparable PCA titer and a total amount of 16.5 ± 1.1 g PCA could be achieved when d-glucose and d-xylose were combined as orthogonal carbon substrates for biocatalyst provision and product synthesis, respectively. Downstream processing of PCA was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. This resulted in a maximum final purity of 95.4%. The established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks.
LB  - PUB:(DE-HGF)16
C6  - 34343343
UR  - <Go to ISI:>//WOS:000686279100001
DO  - DOI:10.1002/bit.27909
UR  - https://juser.fz-juelich.de/record/897250
ER  -