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@ARTICLE{Labib:897250,
      author       = {Labib, Mohamed and Görtz, Jonas and Brüsseler, Christian
                      and Kallscheuer, Nicolai and Gätgens, Jochem and Jupke,
                      Andreas and Marienhagen, Jan and Noack, Stephan},
      title        = {{M}etabolic and process engineering for microbial
                      production of protocatechuate with {C}orynebacterium
                      glutamicum},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {118},
      number       = {11},
      issn         = {1097-0290},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2021-03712},
      pages        = {bit.27909},
      year         = {2021},
      abstract     = {3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic
                      compound naturally found in edible vegetables and medicinal
                      herbs. PCA is of high interest in the chemical industry and
                      has wide potential for pharmaceutical applications. We
                      designed and constructed a novel Corynebacterium glutamicum
                      strain to enable the efficient utilization of d-xylose for
                      microbial production of PCA. Shake flask cultivation of the
                      engineered strain showed a maximum PCA titer of
                      62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from
                      d-xylose as the primary carbon and energy source. The
                      corresponding yield was 0.33 C-mol PCA per C-mol d-xylose,
                      which corresponds to $38\%$ of the maximum theoretical
                      yield. Under growth-decoupled bioreactor conditions, a
                      comparable PCA titer and a total amount of
                      16.5 ± 1.1 g PCA could be achieved when d-glucose and
                      d-xylose were combined as orthogonal carbon substrates for
                      biocatalyst provision and product synthesis, respectively.
                      Downstream processing of PCA was realized via
                      electrochemically induced crystallization by taking
                      advantage of the pH-dependent properties of PCA. This
                      resulted in a maximum final purity of $95.4\%.$ The
                      established PCA production process represents a highly
                      sustainable approach, which will serve as a blueprint for
                      the bio-based production of other hydroxybenzoic acids from
                      alternative sugar feedstocks.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34343343},
      UT           = {WOS:000686279100001},
      doi          = {10.1002/bit.27909},
      url          = {https://juser.fz-juelich.de/record/897250},
}