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| Hauptseite > Publikationsdatenbank > Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum > print |
| Hauptseite > Publikationsdatenbank > Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum > print |
| 001 | 897250 | ||
| 005 | 20220930130327.0 | ||
| 024 | 7 | _ | |a 10.1002/bit.27909 |2 doi |
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| 100 | 1 | _ | |a Labib, Mohamed |0 P:(DE-Juel1)169979 |b 0 |
| 245 | _ | _ | |a Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum |
| 260 | _ | _ | |a New York, NY [u.a.] |c 2021 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a 3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of high interest in the chemical industry and has wide potential for pharmaceutical applications. We designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d-xylose for microbial production of PCA. Shake flask cultivation of the engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L−1) from d-xylose as the primary carbon and energy source. The corresponding yield was 0.33 C-mol PCA per C-mol d-xylose, which corresponds to 38% of the maximum theoretical yield. Under growth-decoupled bioreactor conditions, a comparable PCA titer and a total amount of 16.5 ± 1.1 g PCA could be achieved when d-glucose and d-xylose were combined as orthogonal carbon substrates for biocatalyst provision and product synthesis, respectively. Downstream processing of PCA was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. This resulted in a maximum final purity of 95.4%. The established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks. |
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| 700 | 1 | _ | |a Görtz, Jonas |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Brüsseler, Christian |0 P:(DE-Juel1)166290 |b 2 |
| 700 | 1 | _ | |a Kallscheuer, Nicolai |0 P:(DE-Juel1)157678 |b 3 |
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| 700 | 1 | _ | |a Marienhagen, Jan |0 P:(DE-Juel1)144031 |b 6 |
| 700 | 1 | _ | |a Noack, Stephan |0 P:(DE-Juel1)129050 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/bit.27909 |g p. bit.27909 |0 PERI:(DE-600)1480809-2 |n 11 |p bit.27909 |t Biotechnology & bioengineering |v 118 |y 2021 |x 1097-0290 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/897250/files/bit.27909-1.pdf |y OpenAccess |
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