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@ARTICLE{Siedentop:897391,
      author       = {Siedentop, Regine and Claaßen, Christiane and Rother,
                      Dörte and Lütz, Stephan and Rosenthal, Katrin},
      title        = {{G}etting the {M}ost {O}ut of {E}nzyme {C}ascades:
                      {S}trategies to {O}ptimize {I}n {V}itro {M}ulti-{E}nzymatic
                      {R}eactions},
      journal      = {Catalysts},
      volume       = {11},
      number       = {10},
      issn         = {2073-4344},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2021-03757},
      pages        = {1183 -},
      year         = {2021},
      abstract     = {In vitro enzyme cascades possess great benefits, such as
                      their synthetic capabilities for complex molecules, no need
                      for intermediate isolation, and the shift of unfavorable
                      equilibria towards the products. Their performance, however,
                      can be impaired by, for example, destabilizing or inhibitory
                      interactions between the cascade components or incongruous
                      reaction conditions. The optimization of such systems is
                      therefore often inevitable but not an easy task. Many
                      parameters such as the design of the synthesis route, the
                      choice of enzymes, reaction conditions, or process design
                      can alter the performance of an in vitro enzymatic cascade.
                      Many strategies to tackle this complex task exist, ranging
                      from experimental to in silico approaches and combinations
                      of both. This review collates examples of various
                      optimization strategies and their success. The feasibility
                      of optimization goals, the influence of certain parameters
                      and the usage of algorithm-based optimizations are
                      discussed.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000713311300001},
      doi          = {10.3390/catal11101183},
      url          = {https://juser.fz-juelich.de/record/897391},
}