TY  - JOUR
AU  - Fricke, Philipp Moritz
AU  - Klemm, Angelika
AU  - Bott, Michael
AU  - Polen, Tino
TI  - On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases
JO  - Applied microbiology and biotechnology
VL  - 105
IS  - 9
SN  - 1432-0614
CY  - New York
PB  - Springer
M1  - FZJ-2021-03761
SP  - 3423 - 3456
PY  - 2021
N1  - Biotechnologie 1
AB  - Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is accompanied by growing demands for metabolic engineering of AAB to take advantage of their properties and to improve their biomanufacturing efficiencies. Controlled expression of target genes is key to fundamental and applied microbiological research. In order to get an overview of expression systems and their applications in AAB, we carried out a comprehensive literature search using the Web of Science Core Collection database. The Acetobacteraceae family currently comprises 49 genera. We found overall 6097 publications related to one or more AAB genera since 1973, when the first successful recombinant DNA experiments in Escherichia coli have been published. The use of plasmids in AAB began in 1985 and till today was reported for only nine out of the 49 AAB genera currently described. We found at least five major expression plasmid lineages and a multitude of further expression plasmids, almost all enabling only constitutive target gene expression. Only recently, two regulatable expression systems became available for AAB, an N-acyl homoserine lactone (AHL)-inducible system for Komagataeibacter rhaeticus and an l-arabinose-inducible system for Gluconobacter oxydans. Thus, after 35 years of constitutive target gene expression in AAB, we now have the first regulatable expression systems for AAB in hand and further regulatable expression systems for AAB can be expected.
LB  - PUB:(DE-HGF)16
C6  - pmid:33856535
UR  - <Go to ISI:>//WOS:000640472900002
DO  - DOI:10.1007/s00253-021-11269-z
UR  - https://juser.fz-juelich.de/record/897395
ER  -