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@ARTICLE{Fricke:897395,
author = {Fricke, Philipp Moritz and Klemm, Angelika and Bott,
Michael and Polen, Tino},
title = {{O}n the way toward regulatable expression systems in
acetic acid bacteria: target gene expression and use cases},
journal = {Applied microbiology and biotechnology},
volume = {105},
number = {9},
issn = {1432-0614},
address = {New York},
publisher = {Springer},
reportid = {FZJ-2021-03761},
pages = {3423 - 3456},
year = {2021},
note = {Biotechnologie 1},
abstract = {Acetic acid bacteria (AAB) are valuable biocatalysts for
which there is growing interest in understanding their
basics including physiology and biochemistry. This is
accompanied by growing demands for metabolic engineering of
AAB to take advantage of their properties and to improve
their biomanufacturing efficiencies. Controlled expression
of target genes is key to fundamental and applied
microbiological research. In order to get an overview of
expression systems and their applications in AAB, we carried
out a comprehensive literature search using the Web of
Science Core Collection database. The Acetobacteraceae
family currently comprises 49 genera. We found overall 6097
publications related to one or more AAB genera since 1973,
when the first successful recombinant DNA experiments in
Escherichia coli have been published. The use of plasmids in
AAB began in 1985 and till today was reported for only nine
out of the 49 AAB genera currently described. We found at
least five major expression plasmid lineages and a multitude
of further expression plasmids, almost all enabling only
constitutive target gene expression. Only recently, two
regulatable expression systems became available for AAB, an
N-acyl homoserine lactone (AHL)-inducible system for
Komagataeibacter rhaeticus and an l-arabinose-inducible
system for Gluconobacter oxydans. Thus, after 35 years of
constitutive target gene expression in AAB, we now have the
first regulatable expression systems for AAB in hand and
further regulatable expression systems for AAB can be
expected.},
cin = {IBG-1},
ddc = {570},
cid = {I:(DE-Juel1)IBG-1-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33856535},
UT = {WOS:000640472900002},
doi = {10.1007/s00253-021-11269-z},
url = {https://juser.fz-juelich.de/record/897395},
}