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@ARTICLE{Willuweit:897431,
author = {Willuweit, Antje and Schöneck, Michael and Schemmert,
Sarah and Lohmann, Philipp and Bremen, Saskia and Honold,
Dominik and Burda, Nicole and Jiang, Nan and Beer, Simone
and Ermert, Johannes and Willbold, Dieter and Shah, N. Jon
and Langen, Karl-Josef},
title = {{C}omparison of the {A}myloid {L}oad in the {B}rains of
{T}wo {T}ransgenic {A}lzheimer’s {D}isease {M}ouse
{M}odels {Q}uantified by {F}lorbetaben {P}ositron {E}mission
{T}omography},
journal = {Frontiers in neuroscience},
volume = {15},
issn = {1662-453X},
address = {Lausanne},
publisher = {Frontiers Research Foundation},
reportid = {FZJ-2021-03785},
pages = {699926},
year = {2021},
abstract = {Alzheimer’s disease (AD) is characterized by formation of
amyloid plaques and neurofibrillary tangles in the brain,
which can be mimicked by transgenic mouse models. Here, we
report on the characterization of amyloid load in the brains
of two transgenic amyloidosis models using positron emission
tomography (PET) with florbetaben (FBB), an 18F-labeled
amyloid PET tracer routinely used in AD patients. Young,
middle-aged, and old homozygous APP/PS1 mice (ARTE10), old
hemizygous APPswe/PS1ΔE9, and old wild-type control mice
were subjected to FBB PET using a small animal PET/computed
tomography scanner. After PET, brains were excised, and ex
vivo autoradiography was performed. Plaque pathology was
verified on brain sections with histological methods.
Amyloid plaque load increased progressively with age in the
cortex and hippocampus of ARTE10 mice, which could be
detected with both in vivo FBB PET and ex vivo
autoradiography. FBB retention showed significant
differences to wild-type controls already at 9 months of age
by both in vivo and ex vivo analyses. An excellent
correlation between data derived from PET and
autoradiography could be obtained (rPearson = 0.947, p <
0.0001). Although amyloid load detected by FBB in the brains
of old APPswe/PS1ΔE9 mice was as low as values obtained
with young ARTE10 mice, statistically significant
discrimination to wild-type animals was reached (p < 0.01).
In comparison to amyloid burden quantified by histological
analysis, FBB retention correlated best with total plaque
load and number of congophilic plaques in the brains of both
mouse models. In conclusion, the homozygous ARTE10 mouse
model showed superior properties over APPswe/PS1ΔE9 mice
for FBB small animal amyloid PET imaging. The absolute
amount of congophilic dense-cored plaques seems to be the
decisive factor for feasibility of amyloidosis models for
amyloid PET analysis.},
cin = {INM-4 / INM-11 / JARA-BRAIN / IBI-7 / INM-2 / INM-5},
ddc = {610},
cid = {I:(DE-Juel1)INM-4-20090406 / I:(DE-Juel1)INM-11-20170113 /
I:(DE-Juel1)VDB1046 / I:(DE-Juel1)IBI-7-20200312 /
I:(DE-Juel1)INM-2-20090406 / I:(DE-Juel1)INM-5-20090406},
pnm = {5253 - Neuroimaging (POF4-525) / 5244 - Information
Processing in Neuronal Networks (POF4-524)},
pid = {G:(DE-HGF)POF4-5253 / G:(DE-HGF)POF4-5244},
typ = {PUB:(DE-HGF)16},
pubmed = {34671235},
UT = {WOS:000717045000001},
doi = {10.3389/fnins.2021.699926},
url = {https://juser.fz-juelich.de/record/897431},
}