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@ARTICLE{Abraham:901833,
      author       = {Abraham, Jella-Andrea and Blaschke, Stefan and Tarazi,
                      Samar and Dreissen, Georg and Vay, Sabine U. and Schroeter,
                      Michael and Fink, Gereon R. and Merkel, Rudolf and Rueger,
                      Maria A. and Hoffmann, Bernd},
      title        = {{NSC}s {U}nder {S}train—{U}nraveling the
                      {M}echanoprotective {R}ole of {D}ifferentiating {A}strocytes
                      in a {C}yclically {S}tretched {C}oculture {W}ith
                      {D}ifferentiating {N}eurons},
      journal      = {Frontiers in cellular neuroscience},
      volume       = {15},
      issn         = {1662-5102},
      address      = {Lausanne},
      publisher    = {Frontiers Research Foundation},
      reportid     = {FZJ-2021-03854},
      pages        = {706585},
      year         = {2021},
      abstract     = {The neural stem cell (NSC) niche is a highly vascularized
                      microenvironment that supplies stem cells with relevant
                      biological and chemical cues. However, the NSCs’ proximity
                      to the vasculature also means that the NSCs are subjected to
                      permanent tissue deformation effected by the vessels’
                      heartbeat-induced pulsatile movements. Cultivating NSCs
                      under common culture conditions neglects the—yet
                      unknown—influence of this cyclic mechanical strain on
                      neural stem cells. Under the hypothesis that pulsatile
                      strain should affect essential NSC functions, a cyclic
                      uniaxial strain was applied under biomimetic conditions
                      using an in-house developed stretching system based on
                      cross-linked polydimethylsiloxane (PDMS) elastomer. While
                      lineage commitment remained unaffected by cyclic
                      deformation, strain affected NSC quiescence and cytoskeletal
                      organization. Unexpectedly, cyclically stretched stem cells
                      aligned in stretch direction, a phenomenon unknown for other
                      types of cells in the mammalian organism. The same effect
                      was observed for young astrocytes differentiating from NSCs.
                      In contrast, young neurons differentiating from NSCs did not
                      show mechanoresponsiveness. The exceptional orientation of
                      NSCs and young astrocytes in the stretch direction was
                      blocked upon RhoA activation and went along with a lack of
                      stress fibers. Compared to postnatal astrocytes and mature
                      neurons, NSCs and their young progeny displayed
                      characteristic and distinct mechanoresponsiveness. Data
                      suggest a protective role of young astrocytes in mixed
                      cultures of differentiating neurons and astrocytes by
                      mitigating the mechanical stress of pulsatile strain on
                      developing neurons.},
      cin          = {INM-3 / IBI-2},
      ddc          = {610},
      cid          = {I:(DE-Juel1)INM-3-20090406 / I:(DE-Juel1)IBI-2-20200312},
      pnm          = {5251 - Multilevel Brain Organization and Variability
                      (POF4-525) / 5242 - Information Storage and Processing in
                      the Cell Nucleus (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5251 / G:(DE-HGF)POF4-5242},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34630042},
      UT           = {WOS:000704579500001},
      doi          = {10.3389/fncel.2021.706585},
      url          = {https://juser.fz-juelich.de/record/901833},
}