%0 Journal Article
%A Kappauf, Katrin
%A Majstorovic, Nikola
%A Agarwal, Seema
%A Rother, Dörte
%A Claaßen, Christiane
%T Modulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels
%J ChemBioChem
%V 22
%N 24
%@ 1439-7633
%C Weinheim
%I Wiley-VCH
%M FZJ-2021-03862
%P  3452-3461
%D 2021
%X Smart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature-type poly N-acryloyl glycinamide (pNAGA) hydrogels as a smart matrix for the amine transaminase from Bacillus megaterium (BmTA). Physical entrapment of BmTA in pNAGA hydrogels results in high immobilization efficiency (>89 %) and high activity (97 %). The temperature-sensitiveness of pNAGA is preserved upon immobilization of BmTA and shows a gradual deswelling upon temperature reduction. While enzyme activity is mainly controlled by temperature, deactivation tended to be higher for immobilized BmTA (≈62–68 %) than for free BmTA (≈44 %), suggesting a deactivating effect due to deswelling of the pNAGA gel. Although the deactivation in response to hydrogel deswelling is not yet suitable for controlling enzyme activity sufficiently, it is nevertheless a good starting point for further optimization.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34596326
%U <Go to ISI:>//WOS:000706751900001
%R 10.1002/cbic.202100427
%U https://juser.fz-juelich.de/record/901843