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000901843 1001_ $$0P:(DE-Juel1)179370$$aKappauf, Katrin$$b0
000901843 245__ $$aModulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels
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000901843 520__ $$aSmart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature-type poly N-acryloyl glycinamide (pNAGA) hydrogels as a smart matrix for the amine transaminase from Bacillus megaterium (BmTA). Physical entrapment of BmTA in pNAGA hydrogels results in high immobilization efficiency (>89 %) and high activity (97 %). The temperature-sensitiveness of pNAGA is preserved upon immobilization of BmTA and shows a gradual deswelling upon temperature reduction. While enzyme activity is mainly controlled by temperature, deactivation tended to be higher for immobilized BmTA (≈62–68 %) than for free BmTA (≈44 %), suggesting a deactivating effect due to deswelling of the pNAGA gel. Although the deactivation in response to hydrogel deswelling is not yet suitable for controlling enzyme activity sufficiently, it is nevertheless a good starting point for further optimization.
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000901843 7001_ $$0P:(DE-HGF)0$$aMajstorovic, Nikola$$b1
000901843 7001_ $$0P:(DE-HGF)0$$aAgarwal, Seema$$b2
000901843 7001_ $$0P:(DE-Juel1)144643$$aRother, Dörte$$b3
000901843 7001_ $$0P:(DE-Juel1)176533$$aClaaßen, Christiane$$b4$$eCorresponding author
000901843 773__ $$0PERI:(DE-600)2020469-3$$a10.1002/cbic.202100427$$gp. cbic.202100427$$n24$$p 3452-3461$$tChemBioChem$$v22$$x1439-7633$$y2021
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