TY  - JOUR
AU  - Kappauf, Katrin
AU  - Majstorovic, Nikola
AU  - Agarwal, Seema
AU  - Rother, Dörte
AU  - Claaßen, Christiane
TI  - Modulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels
JO  - ChemBioChem
VL  - 22
IS  - 24
SN  - 1439-7633
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2021-03862
SP  -  3452-3461
PY  - 2021
AB  - Smart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature-type poly N-acryloyl glycinamide (pNAGA) hydrogels as a smart matrix for the amine transaminase from Bacillus megaterium (BmTA). Physical entrapment of BmTA in pNAGA hydrogels results in high immobilization efficiency (>89 %) and high activity (97 %). The temperature-sensitiveness of pNAGA is preserved upon immobilization of BmTA and shows a gradual deswelling upon temperature reduction. While enzyme activity is mainly controlled by temperature, deactivation tended to be higher for immobilized BmTA (≈62–68 %) than for free BmTA (≈44 %), suggesting a deactivating effect due to deswelling of the pNAGA gel. Although the deactivation in response to hydrogel deswelling is not yet suitable for controlling enzyme activity sufficiently, it is nevertheless a good starting point for further optimization.
LB  - PUB:(DE-HGF)16
C6  - pmid:34596326
UR  - <Go to ISI:>//WOS:000706751900001
DO  - DOI:10.1002/cbic.202100427
UR  - https://juser.fz-juelich.de/record/901843
ER  -