% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kappauf:901843,
      author       = {Kappauf, Katrin and Majstorovic, Nikola and Agarwal, Seema
                      and Rother, Dörte and Claaßen, Christiane},
      title        = {{M}odulation of transaminase activity by encapsulation in
                      temperature‐sensitive poly({N}‐acryloyl glycinamide)
                      hydrogels},
      journal      = {ChemBioChem},
      volume       = {22},
      number       = {24},
      issn         = {1439-7633},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2021-03862},
      pages        = {3452-3461},
      year         = {2021},
      abstract     = {Smart hydrogels hold much potential for biocatalysis, not
                      only for the immobilization of enzymes, but also for the
                      control of enzyme activity. We investigated upper critical
                      solution temperature-type poly N-acryloyl glycinamide
                      (pNAGA) hydrogels as a smart matrix for the amine
                      transaminase from Bacillus megaterium (BmTA). Physical
                      entrapment of BmTA in pNAGA hydrogels results in high
                      immobilization efficiency $(>89 \%)$ and high activity
                      $(97 \%).$ The temperature-sensitiveness of pNAGA is
                      preserved upon immobilization of BmTA and shows a gradual
                      deswelling upon temperature reduction. While enzyme activity
                      is mainly controlled by temperature, deactivation tended to
                      be higher for immobilized BmTA $(≈62–68 \%)$ than for
                      free BmTA $(≈44 \%),$ suggesting a deactivating effect
                      due to deswelling of the pNAGA gel. Although the
                      deactivation in response to hydrogel deswelling is not yet
                      suitable for controlling enzyme activity sufficiently, it is
                      nevertheless a good starting point for further
                      optimization.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34596326},
      UT           = {WOS:000706751900001},
      doi          = {10.1002/cbic.202100427},
      url          = {https://juser.fz-juelich.de/record/901843},
}