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| Hauptseite > Publikationsdatenbank > Modulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels > print |
| Hauptseite > Publikationsdatenbank > Modulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels > print |
| 001 | 901843 | ||
| 005 | 20230303201753.0 | ||
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| 100 | 1 | _ | |a Kappauf, Katrin |0 P:(DE-Juel1)179370 |b 0 |
| 245 | _ | _ | |a Modulation of transaminase activity by encapsulation in temperature‐sensitive poly(N‐acryloyl glycinamide) hydrogels |
| 260 | _ | _ | |a Weinheim |c 2021 |b Wiley-VCH |
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| 520 | _ | _ | |a Smart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature-type poly N-acryloyl glycinamide (pNAGA) hydrogels as a smart matrix for the amine transaminase from Bacillus megaterium (BmTA). Physical entrapment of BmTA in pNAGA hydrogels results in high immobilization efficiency (>89 %) and high activity (97 %). The temperature-sensitiveness of pNAGA is preserved upon immobilization of BmTA and shows a gradual deswelling upon temperature reduction. While enzyme activity is mainly controlled by temperature, deactivation tended to be higher for immobilized BmTA (≈62–68 %) than for free BmTA (≈44 %), suggesting a deactivating effect due to deswelling of the pNAGA gel. Although the deactivation in response to hydrogel deswelling is not yet suitable for controlling enzyme activity sufficiently, it is nevertheless a good starting point for further optimization. |
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| 700 | 1 | _ | |a Claaßen, Christiane |0 P:(DE-Juel1)176533 |b 4 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/cbic.202100427 |g p. cbic.202100427 |0 PERI:(DE-600)2020469-3 |n 24 |p 3452-3461 |t ChemBioChem |v 22 |y 2021 |x 1439-7633 |
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