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@ARTICLE{Vay:902080,
author = {Vay, Sabine Ulrike and Olschewski, Daniel Navin and
Petereit, Helena and Lange, Felix and Nazarzadeh, Nilufar
and Gross, Elena and Rabenstein, Monika and Blaschke, Stefan
Johannes and Fink, Gereon Rudolf and Schroeter, Michael and
Rueger, Maria Adele},
title = {{O}steopontin regulates proliferation, migration, and
survival of astrocytes depending on their activation
phenotype},
journal = {Journal of neuroscience research},
volume = {9},
number = {11},
issn = {1097-4547},
address = {New York, NY [u.a.]},
publisher = {Wiley-Liss},
reportid = {FZJ-2021-04020},
pages = {2822-2843},
year = {2021},
abstract = {The glycoprotein osteopontin is highly upregulated in
central nervous system (CNS) disorders such as ischemic
stroke. Osteopontin regulates cell growth, cell adhesion,
homeostasis, migration, and survival of various cell types.
Accordingly, osteopontin is considered an essential
regulator of regeneration and repair in the ischemic milieu.
Astrocytes are the most abundant cells in the CNS and play
significant roles in health and disease. Astrocytes are
involved in homeostasis, promote neuroprotection, and
regulate synaptic plasticity. Upon activation, astrocytes
may adopt different phenotypes, termed A1 and A2. The direct
effects of osteopontin on astrocytes, especially in distinct
activation states, are yet unknown. The current study aimed
to elucidate the impact of osteopontin on resting and active
astrocytes. We established an inflammatory in vitro model of
activated (A1) primary astrocytes derived from neonatal
wistar rats by exposure to a distinct combination of
proinflammatory cytokines. To model ischemic stroke in
vitro, astrocytes were subjected to oxygen and glucose
deprivation (OGD) in the presence or absence of osteopontin.
Osteopontin modulated the activation phenotype by
attenuating A1- and restoring A2-marker expression without
compromising the active astrocytes’ immunocompetence.
Osteopontin promoted the proliferation of active and the
migration of resting astrocytes. Following transient OGD,
osteopontin mitigated the delayed ongoing death of primary
astrocytes, promoting their survival. Data suggest that
osteopontin differentially regulates essential functions of
resting and active astrocytes and confirm a significant
regulatory role of osteopontin in an in vitro ischemia
model. Furthermore, the data suggest that osteopontin
constitutes a promising target for experimental therapies
modulating neuroregeneration and repair.},
cin = {INM-3},
ddc = {570},
cid = {I:(DE-Juel1)INM-3-20090406},
pnm = {5251 - Multilevel Brain Organization and Variability
(POF4-525)},
pid = {G:(DE-HGF)POF4-5251},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34510519},
UT = {WOS:000695029500001},
doi = {10.1002/jnr.24954},
url = {https://juser.fz-juelich.de/record/902080},
}